PMID- 10479679
OWN - NLM
STAT- MEDLINE
DCOM- 19991004
LR  - 20191023
IS  - 1529-2401 (Electronic)
IS  - 0270-6474 (Print)
IS  - 0270-6474 (Linking)
VI  - 19
IP  - 18
DP  - 1999 Sep 15
TI  - Differential expression of brain-derived neurotrophic factor, neurotrophin-3, and 
      neurotrophin-4/5 in the adult rat spinal cord: regulation by the glutamate 
      receptor agonist kainic acid.
PG  - 7757-69
AB  - Previous in vitro studies indicate that select members of the neurotrophin gene 
      family, namely brain-derived neurotrophic factor (BDNF), neurotrophin-3 (NT-3), 
      and neurotrophin-4/5 (NT-4/5), contribute to survival and differentiation of 
      spinal cord motoneurons. To investigate the potential roles of these factors in 
      the adult spinal cord, we examined their cellular localization and regulation 
      after systemic exposure to an excitotoxic stimulus, kainic acid (KA). Of the 
      neurotrophins examined, NT-4/5 mRNA was most robustly expressed in the 
      lumbosacral spinal cord of the normal adult rat, including expression by neurons 
      throughout the gray matter, and in a subpopulation of white and gray matter glia. 
      Both BDNF and NT-3 mRNAs were also densely expressed by alpha motoneurons of 
      lamina IX, but were detected at lower levels elsewhere in the gray matter. NT-3 
      mRNA was additionally expressed by spinal cord glia, but was less widespread 
      compared to NT-4/5. In response to systemic administration of KA, NT-4/5 and BDNF 
      mRNAs were dramatically upregulated in a spatially and temporally restricted 
      fashion, whereas levels of NT-3 mRNA were unchanged. These results provide strong 
      in vivo evidence to support the idea that BDNF, NT-3, and in particular, NT-4/5, 
      play a role in the normal function of the adult spinal cord. Furthermore, our 
      results indicate that the actions of BDNF and NT-4/5 participate in the response 
      of the cord to excitotoxic stimuli, and that those of NT-4/5 and NT-3 include 
      both neurons and glia.
FAU - Scarisbrick, I A
AU  - Scarisbrick IA
AD  - Department of Biochemistry and Molecular Biology, Mayo Clinic Jacksonville, 
      Jacksonville, Florida 32224, USA.
FAU - Isackson, P J
AU  - Isackson PJ
FAU - Windebank, A J
AU  - Windebank AJ
LA  - eng
GR  - N01-HD-7-3263/HD/NICHD NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Neurosci
JT  - The Journal of neuroscience : the official journal of the Society for 
      Neuroscience
JID - 8102140
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Excitatory Amino Acid Agonists)
RN  - 0 (Nerve Growth Factors)
RN  - 0 (Neuroprotective Agents)
RN  - 0 (Neurotrophin 3)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Glutamate)
RN  - 145172-44-7 (neurotrophin 5)
RN  - P658DCA9XD (neurotrophin 4)
RN  - SIV03811UC (Kainic Acid)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/*genetics
MH  - Excitatory Amino Acid Agonists/*pharmacology
MH  - Gene Expression Regulation/drug effects/*physiology
MH  - Kainic Acid/*pharmacology
MH  - Male
MH  - Nerve Growth Factors/*genetics
MH  - Neuroglia/*metabolism
MH  - Neuronal Plasticity
MH  - Neurons/*metabolism
MH  - Neuroprotective Agents
MH  - Neurotrophin 3
MH  - RNA, Messenger/genetics
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Receptors, Glutamate/*physiology
MH  - Spinal Cord/cytology/drug effects/*metabolism
MH  - Transcription, Genetic/*drug effects
PMC - PMC6782449
EDAT- 1999/09/10 00:00
MHDA- 1999/09/10 00:01
PMCR- 2000/03/15
CRDT- 1999/09/10 00:00
PHST- 1999/09/10 00:00 [pubmed]
PHST- 1999/09/10 00:01 [medline]
PHST- 1999/09/10 00:00 [entrez]
PHST- 2000/03/15 00:00 [pmc-release]
AID - 3430 [pii]
AID - 10.1523/JNEUROSCI.19-18-07757.1999 [doi]
PST - ppublish
SO  - J Neurosci. 1999 Sep 15;19(18):7757-69. doi: 10.1523/JNEUROSCI.19-18-07757.1999.