PMID- 10482540
OWN - NLM
STAT- MEDLINE
DCOM- 19991012
LR  - 20200724
IS  - 0022-538X (Print)
IS  - 1098-5514 (Electronic)
IS  - 0022-538X (Linking)
VI  - 73
IP  - 10
DP  - 1999 Oct
TI  - Induction and maintenance of autonomous flock house virus RNA1 replication.
PG  - 7933-42
AB  - The nodavirus flock house virus (FHV) has a bipartite, positive-sense, RNA genome 
      that encodes the catalytic subunit of the RNA replicase and the viral capsid 
      protein precursor on separate genomic segments (RNA1 and RNA2, respectively). 
      RNA1 can replicate autonomously when transfected into permissive cells, allowing 
      study of the kinetics of RNA1 replication in the absence of either RNA2 or capsid 
      proteins. However, RNA1 replication ceases ca. 3 days after transfection despite 
      the presence of replication-competent RNA. We examined this inhibition by 
      inducing the expression of RNA1 in cells from a cDNA copy that was under the 
      control of a hormone-regulated RNA polymerase II promoter. This system reproduced 
      the shutoff of RNA replication when DNA-templated primary transcription was 
      turned off. Continued primary transcription partially alleviated the shutoff and 
      maintained the rate of RNA replication for several days at a steady-state level 
      approximately one-third that of the peak rate. After shutoff, RNA replication 
      could be restored by transferring the resulting intracellular RNA to fresh cells 
      or by reinducing primary transcription, indicating that cessation of replication 
      occurred despite the competence of both the viral RNA and the cytoplasmic 
      environment. These data suggest that there is a mechanism by which replication is 
      shut off at late times after transfection, which may reflect the natural endpoint 
      of the replicative cycle.
FAU - Johnson, K L
AU  - Johnson KL
AD  - Department of Microbiology, University of Alabama at Birmingham, Birmingham, 
      Alabama 35294, USA. kyle_johnson@microbio.uab.edu
FAU - Ball, L A
AU  - Ball LA
LA  - eng
GR  - R01 AI018270/AI/NIAID NIH HHS/United States
GR  - R01 AI18270/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Virol
JT  - Journal of virology
JID - 0113724
RN  - 0 (DNA, Complementary)
RN  - 0 (RNA, Viral)
SB  - IM
MH  - Animals
MH  - DNA, Complementary/genetics
MH  - *Genome, Viral
MH  - Insect Viruses/*genetics
MH  - Kinetics
MH  - RNA Viruses/*genetics
MH  - RNA, Viral/*genetics
MH  - Virus Replication/*genetics
PMC - PMC112807
EDAT- 1999/09/11 00:00
MHDA- 1999/09/11 00:01
PMCR- 1999/10/01
CRDT- 1999/09/11 00:00
PHST- 1999/09/11 00:00 [pubmed]
PHST- 1999/09/11 00:01 [medline]
PHST- 1999/09/11 00:00 [entrez]
PHST- 1999/10/01 00:00 [pmc-release]
AID - 0399 [pii]
AID - 10.1128/JVI.73.10.7933-7942.1999 [doi]
PST - ppublish
SO  - J Virol. 1999 Oct;73(10):7933-42. doi: 10.1128/JVI.73.10.7933-7942.1999.