PMID- 10482564
OWN - NLM
STAT- MEDLINE
DCOM- 19991012
LR  - 20200724
IS  - 0022-538X (Print)
IS  - 1098-5514 (Electronic)
IS  - 0022-538X (Linking)
VI  - 73
IP  - 10
DP  - 1999 Oct
TI  - Lack of interleukin-6 (IL-6) enhances susceptibility to infection but does not 
      alter latency or reactivation of herpes simplex virus type 1 in IL-6 knockout 
      mice.
PG  - 8145-51
AB  - The ability of the pleotropic, proinflammatory cytokine interleukin-6 (IL-6) to 
      affect the replication, latency, and reactivation of herpes simplex virus type 1 
      (HSV-1) in cell culture and in IL-6 knockout (KO) mice was studied. In initial 
      studies, we found no effect of exogenous IL-6, monoclonal antibodies to IL-6, or 
      monoclonal antibody to the IL-6 coreceptor, gp130, on HSV-1 replication in vitro 
      by plaque assay or reactivation ex vivo by explant cocultivation of latently 
      infected murine trigeminal ganglia (TG). Compared with the wild-type (WT) mice, 
      the IL-6 KO mice were less able to survive an ocular challenge with 10(5) PFU of 
      HSV-1 (McKrae) (40% survival of WT and 7% survival KO mice; P = 0.01). There was 
      a sixfold higher 50% lethal dose of HSV-1 in WT than IL-6 KO mice (1.7 x 10(4) 
      and 2.7 x 10(3) PFU, respectively). No differences were observed in titers of 
      virus recovered from the eyes, TG, or brains or in the rates of virus 
      reactivation by explant cocultivation of TG from latently infected WT or KO mice. 
      Exposure of latently infected mice to UV light resulted in comparable rates of 
      reactivation and in the proportions of WT and KO animals experiencing 
      reactivation. Moreover, quantitative PCR assays showed nearly identical numbers 
      of HSV-1 genomes in latently infected WT and IL-6 KO mice. These studies indicate 
      that while IL-6 plays a role in the protection of mice from lethal HSV infection, 
      it does not substantively influence HSV replication, spread to the nervous 
      system, establishment of latency, or reactivation.
FAU - LeBlanc, R A
AU  - LeBlanc RA
AD  - Medical Virology Section, Laboratory of Clinical Investigation, National 
      Institutes of Health, Bethesda, Maryland 20892-1888, USA.
FAU - Pesnicak, L
AU  - Pesnicak L
FAU - Cabral, E S
AU  - Cabral ES
FAU - Godleski, M
AU  - Godleski M
FAU - Straus, S E
AU  - Straus SE
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Virol
JT  - Journal of virology
JID - 0113724
RN  - 0 (Interleukin-6)
SB  - IM
MH  - Animals
MH  - Genetic Predisposition to Disease
MH  - Herpes Simplex/*genetics/*virology
MH  - Herpesvirus 1, Human/*physiology
MH  - Interleukin-6/*genetics
MH  - Mice
MH  - Mice, Knockout
MH  - Virus Activation/*genetics
MH  - Virus Latency/*genetics
PMC - PMC112831
EDAT- 1999/09/11 00:00
MHDA- 1999/09/11 00:01
PMCR- 1999/10/01
CRDT- 1999/09/11 00:00
PHST- 1999/09/11 00:00 [pubmed]
PHST- 1999/09/11 00:01 [medline]
PHST- 1999/09/11 00:00 [entrez]
PHST- 1999/10/01 00:00 [pmc-release]
AID - 0878 [pii]
AID - 10.1128/JVI.73.10.8145-8151.1999 [doi]
PST - ppublish
SO  - J Virol. 1999 Oct;73(10):8145-51. doi: 10.1128/JVI.73.10.8145-8151.1999.