PMID- 10482610
OWN - NLM
STAT- MEDLINE
DCOM- 19991012
LR  - 20200724
IS  - 0022-538X (Print)
IS  - 1098-5514 (Electronic)
IS  - 0022-538X (Linking)
VI  - 73
IP  - 10
DP  - 1999 Oct
TI  - Prevalence of varicella-zoster virus DNA in dissociated human trigeminal ganglion 
      neurons and nonneuronal cells.
PG  - 8571-7
AB  - Previous analyses using in situ hybridization alone or together with PCR have 
      yielded conflicting results regarding the cell type in which latent 
      varicella-zoster virus (VZV) resides. We separated human trigeminal ganglia (TG) 
      into neuronal and nonneuronal fractions, followed by primary and nested PCR to 
      quantitate VZV DNA at the single cell level. Both TG from each of eight cadavers 
      were dissociated and separated into neuronal and nonneuronal cell suspensions by 
      differential filtration. Analysis of the neuron fraction (5,000 neurons per 
      sample) revealed VZV DNA in 9 of 16 samples, with copy numbers ranging from 1 to 
      12, whereas only 2 of 16 nonneuronal cell samples were positive for VZV DNA, with 
      1 copy each. Further analysis of 10 samples of 100 neurons and the corresponding 
      nonneuronal cell fractions from each TG of a single subject revealed VZV DNA in 3 
      of 10 samples of the left TG (range, 2 to 5 copies) and in 1 of 10 samples of the 
      right TG (2 copies) but in none of the 20 nonneuronal cell fractions. These data 
      indicate that latent VZV DNA is present primarily, if not exclusively, in 
      neurons, at a frequency of two to five copies per latently infected neuron.
FAU - LaGuardia, J J
AU  - LaGuardia JJ
AD  - Departments of Neurology, University of Colorado Health Sciences Center, Denver, 
      Colorado 80262, USA.
FAU - Cohrs, R J
AU  - Cohrs RJ
FAU - Gilden, D H
AU  - Gilden DH
LA  - eng
GR  - NS 07321/NS/NINDS NIH HHS/United States
GR  - NS 32623/NS/NINDS NIH HHS/United States
GR  - P01 NS032623/NS/NINDS NIH HHS/United States
GR  - AG 06127/AG/NIA NIH HHS/United States
GR  - T32 NS007321/NS/NINDS NIH HHS/United States
GR  - R37 AG006127/AG/NIA NIH HHS/United States
GR  - R01 AG006127/AG/NIA NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Virol
JT  - Journal of virology
JID - 0113724
RN  - 0 (DNA, Viral)
SB  - IM
MH  - Aged
MH  - DNA, Viral/*analysis
MH  - Female
MH  - Herpes Zoster/pathology/*virology
MH  - Herpesvirus 3, Human/genetics/*isolation & purification
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Neurons/*virology
MH  - Trigeminal Ganglion/pathology/*virology
PMC - PMC112877
EDAT- 1999/09/11 00:00
MHDA- 1999/09/11 00:01
PMCR- 1999/10/01
CRDT- 1999/09/11 00:00
PHST- 1999/09/11 00:00 [pubmed]
PHST- 1999/09/11 00:01 [medline]
PHST- 1999/09/11 00:00 [entrez]
PHST- 1999/10/01 00:00 [pmc-release]
AID - 0701 [pii]
AID - 10.1128/JVI.73.10.8571-8577.1999 [doi]
PST - ppublish
SO  - J Virol. 1999 Oct;73(10):8571-7. doi: 10.1128/JVI.73.10.8571-8577.1999.