PMID- 10482987
OWN - NLM
STAT- MEDLINE
DCOM- 19990930
LR  - 20190915
IS  - 0887-6924 (Print)
IS  - 0887-6924 (Linking)
VI  - 13
IP  - 9
DP  - 1999 Sep
TI  - Frequent allelic loss of the RB, D13S319 and D13S25 locus in myeloid malignancies 
      with deletion/translocation at 13q14 of chromosome 13, but not in lymphoid 
      malignancies.
PG  - 1367-73
AB  - In order to identify a commonly deleted region of 13q14 on chromosome 13, we 
      performed fluorescence in situ hybridization (FISH) on 17 patients with myeloid 
      malignancies and 12 patients with lymphoid leukemia/lymphoma who exhibited either 
      deletion or translocation at 13q14. Three cosmid probes (RB, D13S319 and D13S25) 
      hybridizing to sequences on 13q14 were used. Fourteen of the 17 patients with 
      myeloid malignancies (82.4%) exhibited allelic loss at the RB, D13S319 and D13S25 
      locus, whereas only three of the 12 patients with lymphoid malignancies (25.0%) 
      exhibited loss within these loci. These three patients had chronic lymphocytic 
      leukemia (CLL). Six, two and one of the remaining nine lymphoid leukemia/lymphoma 
      patients had breakpoints centromeric to the RB gene, telomeric to D13S25 and 
      within the D13S319 locus, respectively. A high frequency of allelic loss was 
      found using these probes in patients with myeloid malignancies, compared to in 
      patients with leukemia in the lymphoid origin, except CLL patients. These results 
      indicate that loss of the RB gene itself or a region between RB and D13S319, 
      which includes commonly deleted loci, may play an important role in myeloid 
      leukemogenesis.
FAU - Tanaka, K
AU  - Tanaka K
AD  - Department of Cancer Cytogenetics, Research Institute for Radiation Biology and 
      Medicine, Hiroshima University, Japan.
FAU - Arif, M
AU  - Arif M
FAU - Eguchi, M
AU  - Eguchi M
FAU - Guo, S X
AU  - Guo SX
FAU - Hayashi, Y
AU  - Hayashi Y
FAU - Asaoku, H
AU  - Asaoku H
FAU - Kyo, T
AU  - Kyo T
FAU - Dohy, H
AU  - Dohy H
FAU - Kamada, N
AU  - Kamada N
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Leukemia
JT  - Leukemia
JID - 8704895
RN  - 0 (DNA Probes)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Alleles
MH  - *Chromosome Deletion
MH  - Chromosome Mapping
MH  - *Chromosomes, Human, Pair 13
MH  - DNA Probes
MH  - Female
MH  - *Genes, Retinoblastoma
MH  - Humans
MH  - Leukemia, Lymphocytic, Chronic, B-Cell/genetics
MH  - Leukemia, Lymphoid/genetics
MH  - Leukemia, Myeloid/*genetics
MH  - Male
MH  - Middle Aged
MH  - Myelodysplastic Syndromes/*genetics
MH  - *Translocation, Genetic
EDAT- 1999/09/14 00:00
MHDA- 1999/09/14 00:01
CRDT- 1999/09/14 00:00
PHST- 1999/09/14 00:00 [pubmed]
PHST- 1999/09/14 00:01 [medline]
PHST- 1999/09/14 00:00 [entrez]
AID - 10.1038/sj.leu.2401482 [doi]
PST - ppublish
SO  - Leukemia. 1999 Sep;13(9):1367-73. doi: 10.1038/sj.leu.2401482.