PMID- 10483526
OWN - NLM
STAT- MEDLINE
DCOM- 19991015
LR  - 20191024
IS  - 0190-2148 (Print)
IS  - 0190-2148 (Linking)
VI  - 25
IP  - 5
DP  - 1999 Jul-Aug
TI  - Discordant pulmonary proinflammatory cytokine expression during acute hyperoxia 
      in the newborn rabbit.
PG  - 443-65
AB  - Newborn animals are resistant to oxygen toxicity. To investigate this phenomenon, 
      the proinflammatory cytokines interleukin (IL)-1 beta, IL-8, and monocyte 
      chemoattractant protein-1 (MCP-1) were measured during newborn rabbit hyperoxic 
      lung injury. Pups were exposed to > 95% O2 for 8-9 days, followed by 60% O2 until 
      36 days of age. Lung lavage fluid, RNA, and tissue sections were collected at 0, 
      2, 4, 6, 8, 10, 12, 14, 22, and 36 days. Acute inflammation occurred by 6-10 days 
      of hyperoxia, and fibrosis by 22 days. Northern hybridization of lung homogenates 
      from hyperoxia-exposed pups showed elevated MCP-1 and IL-8 mRNA expression at 6 
      and 10 days, respectively, compared to age-matched, air-exposed controls. Lavage 
      fluid IL-8 protein also peaked at 10 days, and was strongly correlated to 
      neutrophil numbers in lavage. In situ hybridization revealed elevated IL-1 beta 
      mRNA in macrophages, alveolar epithelial and interstitial cells at 2-10 days, 
      elevated MCP-1 mRNA in similar cell types at 4-8 days, and elevated IL-8 mRNA in 
      these cells and neutrophils at 4-10 days. IL-1 beta and IL-8 expression peaked 
      during peak inflammation, whereas peak MCP-1 expression preceded macrophage 
      influx. Comparing newborn and adult animals' chemokine response may help explain 
      their differences in hyperoxia susceptibility.
FAU - D'Angio, C T
AU  - D'Angio CT
AD  - Department of Pediatrics (Neonatology), Strong Children's Research Center, 
      University of Rochester, New York, USA. carl_dangio@urmc.rochester.edu
FAU - LoMonaco, M B
AU  - LoMonaco MB
FAU - Chaudhry, S A
AU  - Chaudhry SA
FAU - Paxhia, A
AU  - Paxhia A
FAU - Ryan, R M
AU  - Ryan RM
LA  - eng
GR  - 5F32HL-09022/HL/NHLBI NIH HHS/United States
GR  - HL-02630/HL/NHLBI NIH HHS/United States
GR  - HL-36543/HL/NHLBI NIH HHS/United States
GR  - etc.
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Exp Lung Res
JT  - Experimental lung research
JID - 8004944
RN  - 0 (Chemokine CCL2)
RN  - 0 (Interleukin-1)
RN  - 0 (Interleukin-8)
RN  - 0 (RNA, Messenger)
RN  - S88TT14065 (Oxygen)
SB  - IM
MH  - Animals
MH  - Animals, Newborn/*metabolism
MH  - Blotting, Northern
MH  - Bronchoalveolar Lavage Fluid/chemistry
MH  - Cell Count
MH  - Chemokine CCL2/genetics/*metabolism
MH  - Disease Models, Animal
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Epithelial Cells/metabolism
MH  - Hyperoxia/*metabolism
MH  - In Situ Hybridization
MH  - Interleukin-1/genetics/*metabolism
MH  - Interleukin-8/genetics/*metabolism
MH  - Lung/metabolism/pathology
MH  - Macrophages, Alveolar/cytology/metabolism
MH  - Oxygen/blood
MH  - Pulmonary Alveoli/cytology/metabolism
MH  - Pulmonary Fibrosis/metabolism/pathology
MH  - RNA, Messenger/analysis/metabolism
MH  - Rabbits
EDAT- 1999/09/14 00:00
MHDA- 1999/09/14 00:01
CRDT- 1999/09/14 00:00
PHST- 1999/09/14 00:00 [pubmed]
PHST- 1999/09/14 00:01 [medline]
PHST- 1999/09/14 00:00 [entrez]
AID - 10.1080/019021499270187 [doi]
PST - ppublish
SO  - Exp Lung Res. 1999 Jul-Aug;25(5):443-65. doi: 10.1080/019021499270187.