PMID- 10484363
OWN - NLM
STAT- MEDLINE
DCOM- 19991028
LR  - 20181018
IS  - 0002-9513 (Print)
IS  - 0002-9513 (Linking)
VI  - 277
IP  - 3
DP  - 1999 Sep
TI  - Metabolic control analysis of insulin-stimulated glucose disposal in rat skeletal 
      muscle.
PG  - E505-12
LID - 10.1152/ajpendo.1999.277.3.E505 [doi]
AB  - Metabolic control analysis was used to calculate the distributed control of 
      insulin-stimulated skeletal muscle glucose disposal in awake rats. Three separate 
      hyperinsulinemic infusion protocols were performed: 1) protocol I was a 
      euglycemic (approximately 6 mM)-hyperinsulinemic (10 mU. kg(-1). min(-1)) clamp, 
      2) protocol II was a hyperglycemic ( approximately 11 mM)-hyperinsulinemic (10 
      mU. kg(-1). min(-1)) clamp, and 3) protocol III was a euglycemic (approximately 6 
      mM)-hyperinsulinemic (10 mU. kg(-1). min(-1))-lipid/heparin (increased plasma 
      free fatty acid) clamp. [1-13C]glucose was administered in all three protocols 
      for a 3-h period, during which time [1-13C]glucose label incorporation into 
      [1-13)]glycogen, [3-13C]lactate, and [3-13C]alanine was detected in the hindlimb 
      of awake rats via 13C-NMR. Combined steady-state and kinetic data were used to 
      calculate rates of glycogen synthesis and glycolysis. Additionally, glucose 
      6-phosphate (G-6-P) was measured in the hindlimb muscles with the use of in vivo 
      31P-NMR during the three infusion protocols. The clamped glucose infusion rates 
      were 31.6 +/- 2.9, 49.7 +/- 1.0, and 24.0 +/- 1.5 mg. kg(-1). min(-1) at 120 min 
      in protocols I-III, respectively. Rates of glycolysis were 62.1 +/- 10.3, 71.6 
      +/- 11.8, and 19.5 +/- 3.6 nmol. g(-1). min(-1) and rates of glycogen synthesis 
      were 125 +/- 15, 224 +/- 23, and 104 +/- 17 nmol. g(-1). min(-1) in protocols 
      I-III, respectively. Insulin-stimulated G-6-P concentrations were 217 +/- 8, 265 
      +/- 12, and 251 +/- 9 nmol/g in protocols I-III, respectively. A top-down 
      approach to metabolic control analysis was used to calculate the distributed 
      control among glucose transport/phosphorylation [GLUT-4/hexokinase (HK)], 
      glycogen synthesis, and glycolysis from the metabolic flux and G-6-P data. The 
      calculated values for the control coefficients (C) of these three metabolic steps 
      (C(J)(GLUT-4/HK) = 0.55 +/- 0.10, C(J)(glycogen syn) = 0.30 +/- 0.06, and 
      C(J)(glycolysis) = 0.15 +/- 0.02; where J is glucose disposal flux, and glycogen 
      syn is glycogen synthesis) indicate that there is shared control of glucose 
      disposal and that glucose transport/phosphorylation is responsible for the 
      majority of control of insulin-stimulated glucose disposal in skeletal muscle.
FAU - Jucker, B M
AU  - Jucker BM
AD  - Department of Internal Medicine, Yale University School of Medicine, New Haven, 
      Connecticut 06520-8020, USA. Jucker@mrclin1.med.yale.edu
FAU - Barucci, N
AU  - Barucci N
FAU - Shulman, G I
AU  - Shulman GI
LA  - eng
GR  - R01 DK040936/DK/NIDDK NIH HHS/United States
GR  - P30-DK-45735/DK/NIDDK NIH HHS/United States
GR  - R01-DK-40936/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Am J Physiol
JT  - The American journal of physiology
JID - 0370511
RN  - 0 (Blood Glucose)
RN  - 0 (Fatty Acids, Nonesterified)
RN  - 0 (Insulin)
RN  - 56-73-5 (Glucose-6-Phosphate)
RN  - 9005-79-2 (Glycogen)
RN  - IY9XDZ35W2 (Glucose)
SB  - IM
MH  - Animals
MH  - Blood Glucose/analysis
MH  - Fatty Acids, Nonesterified/blood
MH  - Glucose/*metabolism
MH  - Glucose Clamp Technique
MH  - Glucose-6-Phosphate/metabolism
MH  - Glycogen/biosynthesis
MH  - Glycolysis
MH  - Insulin/blood/*pharmacology
MH  - Magnetic Resonance Spectroscopy
MH  - Muscle, Skeletal/*drug effects/*metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
EDAT- 1999/09/14 00:00
MHDA- 1999/09/14 00:01
CRDT- 1999/09/14 00:00
PHST- 1999/09/14 00:00 [pubmed]
PHST- 1999/09/14 00:01 [medline]
PHST- 1999/09/14 00:00 [entrez]
AID - 10.1152/ajpendo.1999.277.3.E505 [doi]
PST - ppublish
SO  - Am J Physiol. 1999 Sep;277(3):E505-12. doi: 10.1152/ajpendo.1999.277.3.E505.