PMID- 10484989
OWN - NLM
STAT- MEDLINE
DCOM- 19990928
LR  - 20190816
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 113
IP  - 2
DP  - 1999 Sep
TI  - Deletion 7q in uterine leiomyoma: fluorescence in situ hybridization 
      characterization on primary cytogenetic preparations.
PG  - 183-7
AB  - Deletions of the long arm of chromosome 7 constitute one of the most common 
      clonal chromosomal changes associated with uterine leiomyoma cells. Recently, the 
      molecular cytogenetic refinement of 7q deletions in two myoma-derived cell lines, 
      with the use of a panel of 39 ordered 7q DNA probes corresponding to 87 genetic 
      markers, showed results in line with those obtained by loss of heterozygosity 
      (LOH) analysis. Referring to this panel, we extended fluorescence in situ 
      hybridization (FISH) analysis on primary cytogenetic preparations from three 
      myomas with del(7q), thereby avoiding cell passages. This was fundamental in the 
      maintenance of cells with del(7q) in the two cases showing mosaicism (i.e., the 
      presence of an extra normal clone), which are prone to lose the abnormal clone in 
      the very early passages. The data obtained, together with previously published 
      findings on the two leiomyoma-derived cell lines, indicated a commonly deleted 
      region of 11 cM. If the fact that the presence of normal cells may interfere with 
      LOH analysis is taken into account, the FISH approach seems to be a reliable 
      complementing tool for refining the deletion and analyzing the smallest 
      overlapping region in cases with both normal and del(7q) cells.
FAU - Vanni, R
AU  - Vanni R
AD  - Center for Human Genetics, University of Leuven, Belgium.
FAU - Schoenmakers, E F
AU  - Schoenmakers EF
FAU - Andria, M
AU  - Andria M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
RN  - 0 (DNA Probes)
SB  - IM
MH  - *Chromosome Deletion
MH  - Chromosomes, Artificial, Yeast
MH  - *Chromosomes, Human, Pair 7
MH  - DNA Probes
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Leiomyoma/*genetics
MH  - Uterine Neoplasms/*genetics
EDAT- 1999/09/15 00:00
MHDA- 1999/09/15 00:01
CRDT- 1999/09/15 00:00
PHST- 1999/09/15 00:00 [pubmed]
PHST- 1999/09/15 00:01 [medline]
PHST- 1999/09/15 00:00 [entrez]
AID - S0165460899000254 [pii]
AID - 10.1016/s0165-4608(99)00025-4 [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 1999 Sep;113(2):183-7. doi: 
      10.1016/s0165-4608(99)00025-4.