PMID- 10486052
OWN - NLM
STAT- MEDLINE
DCOM- 19991201
LR  - 20191024
IS  - 0343-8651 (Print)
IS  - 0343-8651 (Linking)
VI  - 39
IP  - 4
DP  - 1999 Oct
TI  - Proteolytic activities of the starch-fermenting ruminal bacterium, Streptococcus 
      bovis.
PG  - 180-6
AB  - The objective of this study was to characterize the extracellular proteolytic 
      activity of Streptococcus bovis. Strains KEG, JB1, NCFB 2476, and K11.21.09.6C 
      produced very similar large molecular weight (160-200 kDa) extracellular 
      proteases that were specifically inhibited by PMSF, a serine protease inhibitor. 
      Further experiments with S. bovis KEG indicated that cultures grown with casein 
      as the sole added N source produced the greatest level of proteolytic activity, 
      and the level of proteolytic activity was independent of growth rate. Clarified 
      ruminal fluid (CRF) decreased proteolytic activity by 54% compared with cultures 
      grown with casein alone, and addition of exogenous peptides and carbohydrates 
      (CHO) to the CRF further reduced the level of proteolytic activity by 44% and 
      52%, respectively. These results suggested that the proteolytic activity of S. 
      bovis KEG was modulated by available N source and that the proteolytic activity 
      was present for reasons other than providing N for growth. The role of S. bovis 
      in ruminal proteolysis requires further definition, but phenotypic similarity 
      among some ruminal strains would suggest a common niche in ruminal proteolysis. 
      The uniformity of proteolytic activities could make S. bovis a prime candidate 
      for manipulation in ruminal proteolysis control strategies.
FAU - Griswold, K E
AU  - Griswold KE
AD  - Department of Animal Sciences, University of Illinois, 1207 W. Gregory Dr., 
      Urbana, IL 61801, USA.
FAU - White, B A
AU  - White BA
FAU - Mackie, R I
AU  - Mackie RI
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Curr Microbiol
JT  - Current microbiology
JID - 7808448
RN  - 0 (Culture Media)
RN  - 0 (Serine Proteinase Inhibitors)
RN  - 9005-25-8 (Starch)
RN  - EC 3.4.- (Endopeptidases)
RN  - N762921K75 (Nitrogen)
SB  - IM
MH  - Animals
MH  - Culture Media
MH  - Electrophoresis, Polyacrylamide Gel/methods
MH  - Endopeptidases/*metabolism
MH  - Nitrogen/metabolism
MH  - Rumen/*microbiology
MH  - Serine Proteinase Inhibitors/pharmacology
MH  - Starch/metabolism
MH  - Streptococcus bovis/*enzymology/growth & development
EDAT- 1999/09/15 00:00
MHDA- 1999/09/15 00:01
CRDT- 1999/09/15 00:00
PHST- 1999/09/15 00:00 [pubmed]
PHST- 1999/09/15 00:01 [medline]
PHST- 1999/09/15 00:00 [entrez]
AID - 2992 [pii]
AID - 10.1007/s002849900442 [doi]
PST - ppublish
SO  - Curr Microbiol. 1999 Oct;39(4):180-6. doi: 10.1007/s002849900442.