PMID- 10486056
OWN - NLM
STAT- MEDLINE
DCOM- 19991201
LR  - 20191024
IS  - 0343-8651 (Print)
IS  - 0343-8651 (Linking)
VI  - 39
IP  - 4
DP  - 1999 Oct
TI  - Trehalose phosphorylase activity and carbohydrate levels during axenic fruiting 
      in three Agaricus bisporus strains.
PG  - 205-10
AB  - Three strains of Agaricus bisporus (B430, 116, and 155.8), which share the 
      ability to form hyphal aggregates on solid media under axenic conditions, were 
      investigated with respect to carbohydrate levels and activities of enzymes 
      involved in their carbon metabolism. The size and macroscopic appearance of the 
      aggregates, when grown on diluted medium, suggest that substrate limitation plays 
      a role in the process of fruiting body development in A. bisporus. The enzymes 
      trehalose phosphorylase (TP), mannitol dehydrogenase (MD), and 
      glucose-6-phosphate dehydrogenase (G6PD) seem to be developmentally regulated, in 
      contrast to hexokinase (HK). Activities of TP (measured in the direction of 
      trehalose degradation), MD, and G6PD were higher in the hyphal aggregates 
      compared with the mycelium, whereas HK activity varied little. In the period 
      preceding the axenic formation of hyphal aggregates, synthesis of trehalose by TP 
      approximately doubled in the mycelium. The carbohydrate levels, which were 
      measured by HPLC, varied in a way similar to their corresponding enzymes. The 
      results indicate synthesis of trehalose in the mycelium of A. bisporus before the 
      hyphal aggregates arise. Subsequently, translocation of the trehalose takes place 
      from the mycelium to the emerging aggregates. In these small aggregates the 
      trehalose is rapidly broken down to yield glucose and glucose-1-phosphate, 
      serving as carbon and energy sources for further growth of the aggregates and for 
      the synthesis of the osmolyte mannitol.
FAU - Wannet, W J
AU  - Wannet WJ
AD  - Department of Microbiology, Faculty of Science, University of Nijmegen, 
      Toernooiveld 1, NL-6525 ED Nijmegen, The Netherlands.
FAU - Aben, E M
AU  - Aben EM
FAU - van der Drift, C
AU  - van der Drift C
FAU - Van Griensven, L J
AU  - Van Griensven LJ
FAU - Vogels, G D
AU  - Vogels GD
FAU - Op den Camp, H J
AU  - Op den Camp HJ
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Curr Microbiol
JT  - Current microbiology
JID - 7808448
RN  - 0 (Culture Media)
RN  - 3OWL53L36A (Mannitol)
RN  - B8WCK70T7I (Trehalose)
RN  - EC 2.4.1.- (Glucosyltransferases)
RN  - EC 2.4.1.64 (alpha,alpha-trehalose phosphorylase)
RN  - IY9XDZ35W2 (Glucose)
SB  - IM
MH  - Agaricus/*enzymology/*growth & development
MH  - *Carbohydrate Metabolism
MH  - Chromatography, High Pressure Liquid
MH  - Culture Media
MH  - Glucose/metabolism
MH  - Glucosyltransferases/*metabolism
MH  - Mannitol/metabolism
MH  - Trehalose/metabolism
EDAT- 1999/09/15 00:00
MHDA- 1999/09/15 00:01
CRDT- 1999/09/15 00:00
PHST- 1999/09/15 00:00 [pubmed]
PHST- 1999/09/15 00:01 [medline]
PHST- 1999/09/15 00:00 [entrez]
AID - 3016 [pii]
AID - 10.1007/s002849900446 [doi]
PST - ppublish
SO  - Curr Microbiol. 1999 Oct;39(4):205-10. doi: 10.1007/s002849900446.