PMID- 10486154
OWN - NLM
STAT- MEDLINE
DCOM- 19991021
LR  - 20171116
IS  - 0008-8749 (Print)
IS  - 0008-8749 (Linking)
VI  - 196
IP  - 1
DP  - 1999 Aug 25
TI  - Interleukin-18 induces interferon-gamma production through NF-kappaB and NFAT 
      activation in murine T helper type 1 cells.
PG  - 41-50
AB  - Interleukin-18 (IL-18) combined with anti-CD3 monoclonal antibody (mAb) induced 
      interferon-gamma (IFN-gamma) production by T helper type 1 (Th1) cells. Neither 
      IL-18 nor anti-CD3 mAb alone induced production of IFN-gamma. Although treatment 
      with IL-18 alone induced full activation of NF-kappaB in Th1 cells, it was not 
      sufficient for the production of IFN-gamma. To examine the importance of 
      NF-kappaB activation in IFN-gamma production, we established Th1 cells which 
      expressed a transdominant IkappaBalpha mutant. In these cells, activation of 
      NF-kappaB and production of IFN-gamma by IL-18 were suppressed. On the other 
      hand, we examined the T cell receptor (TCR)/CD3-mediated signaling pathway. 
      FK506, an inhibitor of NFAT activation, inhibited IFN-gamma production by IL-18 
      without any effect on the NF-kappaB activation. We conclude that dual signaling 
      consisting of IL-18-induced NF-kappaB activation and TCR/CD3-mediated NFAT 
      activation is crucial for IFN-gamma production by IL-18 in murine Th1 cells.
CI  - Copyright 1999 Academic Press.
FAU - Tsuji-Takayama, K
AU  - Tsuji-Takayama K
AD  - Fujisaki Institute, Hayashibara Biochemical Laboratories, Inc., 675-1 Fujisaki, 
      Okayama, 702-8006, Japan.
FAU - Aizawa, Y
AU  - Aizawa Y
FAU - Okamoto, I
AU  - Okamoto I
FAU - Kojima, H
AU  - Kojima H
FAU - Koide, K
AU  - Koide K
FAU - Takeuchi, M
AU  - Takeuchi M
FAU - Ikegami, H
AU  - Ikegami H
FAU - Ohta, T
AU  - Ohta T
FAU - Kurimoto, M
AU  - Kurimoto M
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - Cell Immunol
JT  - Cellular immunology
JID - 1246405
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (CD3 Complex)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (I-kappa B Proteins)
RN  - 0 (Interleukin-18)
RN  - 0 (Isoenzymes)
RN  - 0 (NF-kappa B)
RN  - 0 (NFATC Transcription Factors)
RN  - 0 (NFKBIA protein, human)
RN  - 0 (Nfkbia protein, mouse)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Transcription Factors)
RN  - 139874-52-5 (NF-KappaB Inhibitor alpha)
RN  - 82115-62-6 (Interferon-gamma)
RN  - EC 2.7.11.17 (Calcium-Calmodulin-Dependent Protein Kinases)
RN  - EC 3.1.4.- (Type C Phospholipases)
RN  - EC 3.1.4.3 (Phospholipase C gamma)
RN  - WM0HAQ4WNM (Tacrolimus)
SB  - IM
MH  - Animals
MH  - Antibodies, Monoclonal
MH  - CD3 Complex/immunology
MH  - Calcium-Calmodulin-Dependent Protein Kinases/metabolism
MH  - Cells, Cultured
MH  - DNA-Binding Proteins/genetics/*metabolism
MH  - Humans
MH  - *I-kappa B Proteins
MH  - Interferon-gamma/*biosynthesis
MH  - Interleukin-18/*physiology
MH  - Isoenzymes/metabolism
MH  - Mice
MH  - NF-KappaB Inhibitor alpha
MH  - NF-kappa B/*metabolism
MH  - NFATC Transcription Factors
MH  - *Nuclear Proteins
MH  - Phospholipase C gamma
MH  - Phosphorylation
MH  - Signal Transduction
MH  - Tacrolimus/metabolism/pharmacology
MH  - Th1 Cells/drug effects/*metabolism
MH  - Transcription Factors/*metabolism
MH  - Transformation, Genetic
MH  - Type C Phospholipases/metabolism
EDAT- 1999/09/16 00:00
MHDA- 1999/09/16 00:01
CRDT- 1999/09/16 00:00
PHST- 1999/09/16 00:00 [pubmed]
PHST- 1999/09/16 00:01 [medline]
PHST- 1999/09/16 00:00 [entrez]
AID - S0008-8749(99)91542-3 [pii]
AID - 10.1006/cimm.1999.1542 [doi]
PST - ppublish
SO  - Cell Immunol. 1999 Aug 25;196(1):41-50. doi: 10.1006/cimm.1999.1542.