PMID- 10487690
OWN - NLM
STAT- MEDLINE
DCOM- 19991006
LR  - 20220331
IS  - 0021-972X (Print)
IS  - 0021-972X (Linking)
VI  - 84
IP  - 9
DP  - 1999 Sep
TI  - Metabolism of dihydrotestosterone in human liver: importance of 3alpha- and 
      3beta-hydroxysteroid dehydrogenase.
PG  - 3217-21
AB  - This study compared the enzyme activity of 3alpha-hydroxysteroid dehydrogenase 
      (3alphaHSD) and 3beta-hydroxysteroid dehydrogenase (3betaHSD) in the human liver. 
      3AlphaHSD was found in both microsomal and cytosolic liver fractions. Contrary to 
      that in rat liver, microsomal 3alphaHSD activity was 12-fold higher than 
      cytosolic 3alphaHSD activity, and 3alphaHSD was not inhibited by indomethacin (10 
      micromol/L). The rate of 5alpha-dihydrotestosterone (DHT) reduction to 
      5alpha-androstane-3alpha,17beta-diol (3alphaDIOL) by 3alphaHSD was 2 times higher 
      than the rate of 3alphaDIOL oxidation to DHT. 3BetaHSD was present primarily in 
      the microsomal fraction of the human liver, and the rate of DHT reduction to 
      5alpha-androstane-3beta,17beta-diol (3betaDIOL) by 3betaHSD was 3 times higher 
      than the rate of 3betaHSD oxidation to DHT. When 3alphaHSD and 3betaHSD 
      activities were compared, the rate of DHT reduction by 3betaHSD was 3-fold lower 
      than the rate of DHT reduction by 3alphaHSD. No sex or age differences were found 
      in either 3alphaHSD or 3betaHSD activity. As the activity of DHT-metabolizing 
      enzymes is not sex dependent, the sex differences in plasma levels of 3alphaDIOL 
      glucuronide probably reflect differences in DHT production rather than in DHT 
      metabolism. Comparison of the activities of 3alphaHSD, 3betaHSD, and androgen 
      UDP-glucuronyl transferase suggests that the major pathway of DHT metabolism in 
      human liver involves 3alphaHSD reduction in the liver, followed by subsequent 
      glucuronidation and clearance via the kidney.
FAU - Pirog, E C
AU  - Pirog EC
AD  - Department of Pathology, Cornell University School of Medicine and Veterans 
      Administration Medical Center, Lexington, Kentucky 40536, USA.
FAU - Collins, D C
AU  - Collins DC
LA  - eng
GR  - R01-DK-41879/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Clin Endocrinol Metab
JT  - The Journal of clinical endocrinology and metabolism
JID - 0375362
RN  - 08J2K08A3Y (Dihydrotestosterone)
RN  - 25126-76-5 (Androstane-3,17-diol)
RN  - EC 1.1.- (3-Hydroxysteroid Dehydrogenases)
RN  - EC 1.1.1.50 (3-alpha-Hydroxysteroid Dehydrogenase (B-Specific))
SB  - IM
MH  - 3-Hydroxysteroid Dehydrogenases/*metabolism
MH  - 3-alpha-Hydroxysteroid Dehydrogenase (B-Specific)
MH  - Adult
MH  - Aged
MH  - Androstane-3,17-diol/metabolism
MH  - Dihydrotestosterone/*metabolism
MH  - Female
MH  - Humans
MH  - Kinetics
MH  - Liver/*metabolism
MH  - Male
MH  - Middle Aged
MH  - Reference Values
MH  - Sex Characteristics
EDAT- 1999/09/16 00:00
MHDA- 1999/09/16 00:01
CRDT- 1999/09/16 00:00
PHST- 1999/09/16 00:00 [pubmed]
PHST- 1999/09/16 00:01 [medline]
PHST- 1999/09/16 00:00 [entrez]
AID - 10.1210/jcem.84.9.5963 [doi]
PST - ppublish
SO  - J Clin Endocrinol Metab. 1999 Sep;84(9):3217-21. doi: 10.1210/jcem.84.9.5963.