PMID- 10487840
OWN - NLM
STAT- MEDLINE
DCOM- 19991007
LR  - 20181113
IS  - 0002-9440 (Print)
IS  - 1525-2191 (Electronic)
IS  - 0002-9440 (Linking)
VI  - 155
IP  - 3
DP  - 1999 Sep
TI  - Activation of infiltrating cytotoxic T lymphocytes and lymphoma cell apoptotic 
      rates in gastric MALT lymphomas. Differences between high-grade and low-grade 
      cases.
PG  - 823-9
AB  - In this study, we have characterized infiltrating T lymphocytes from 13 low-grade 
      and 17 high-grade primary gastric MALT lymphomas by immunohistochemistry, with 
      particular regard to the presence, activation, and topographic distribution of 
      cytotoxic effectors. Although the prevalence of CD4+ and CD8+ cells was similar 
      in low- and high-grade lymphomas, higher numbers of TIA-1+ cytotoxic effectors 
      were found in this latter group of cases (11.6 versus 7. 8%; P = 0.004). 
      Activation of CD8+ cytotoxic T lymphocytes (CTLs) was significantly more 
      pronounced in high- than in low-grade lymphomas, as shown by immunostaining for 
      perforin (8.7 versus 4.0%; P = 0.001) and granzyme-B (GrB) (8.7% versus 3.0%; P < 
      0.0001). Of note, CD20/GrB double labeling showed that high-grade lymphomas 
      carried a markedly higher content (about ninefold) of activated CTLs relative to 
      the number of CD20+ lymphoma B cells (0.081 +/- 0.076 versus 0.009 +/- 0.011; P < 
      0.0001). Moreover, high-grade lymphomas showed significantly increased apoptotic 
      rates compared to low-grade cases (5.3 and 1.1% of terminal deoxynucleotidyl 
      transferase-mediated dUTP nick end labeling (TUNEL)-positive cells, respectively; 
      P < 0.0001). In the whole series, the percentage of GrB+ cells and the GrB+/CD20+ 
      ratio showed a strong linear correlation with the number of TUNEL-labeled cells. 
      These findings, together with the frequent colocalization of CTLs and TUNEL+ 
      neoplastic cells, suggested that apoptotic death of lymphoma cells may be due at 
      least in part to the killing by cytotoxic effectors. Our results are consistent 
      with the occurrence of host antitumor cell-mediated immune responses in gastric 
      MALT lymphomas. Moreover, the finding of stronger cytotoxic responses in high- 
      than in low-grade cases is of potential usefulness in the design of more 
      effective therapeutic strategies for the management of these disorders.
FAU - Guidoboni, M
AU  - Guidoboni M
AD  - Division of Experimental Oncology 1,(*) Centro di Riferimento Oncologico, Aviano, 
      Italy Belluno City Hospital, Belluno, Italy.
FAU - Doglioni, C
AU  - Doglioni C
FAU - Laurino, L
AU  - Laurino L
FAU - Boiocchi, M
AU  - Boiocchi M
FAU - Dolcetti, R
AU  - Dolcetti R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Am J Pathol
JT  - The American journal of pathology
JID - 0370502
RN  - 0 (Antigens, CD20)
RN  - 0 (CD56 Antigen)
RN  - 0 (GRB10 protein, human)
RN  - 0 (Membrane Proteins)
RN  - 0 (Poly(A)-Binding Proteins)
RN  - 0 (Proteins)
RN  - 0 (RNA-Binding Proteins)
RN  - 0 (T-Cell Intracellular Antigen-1)
RN  - 0 (TIA1 protein, human)
RN  - 151441-47-3 (GRB10 Adaptor Protein)
SB  - IM
MH  - Antigens, CD20/metabolism
MH  - *Apoptosis
MH  - CD4-CD8 Ratio
MH  - CD56 Antigen/metabolism
MH  - Cell Count
MH  - GRB10 Adaptor Protein
MH  - Humans
MH  - Immunohistochemistry
MH  - In Situ Nick-End Labeling
MH  - Lymphocyte Activation/*immunology
MH  - Lymphocytes, Tumor-Infiltrating/immunology/*metabolism
MH  - Lymphoma, B-Cell, Marginal Zone/*immunology/pathology
MH  - Membrane Proteins/metabolism
MH  - Poly(A)-Binding Proteins
MH  - Proteins/metabolism
MH  - RNA-Binding Proteins/metabolism
MH  - Stomach Neoplasms/*immunology/pathology
MH  - T-Cell Intracellular Antigen-1
MH  - T-Lymphocytes, Cytotoxic/immunology/*metabolism
PMC - PMC1866896
EDAT- 1999/09/17 00:00
MHDA- 1999/09/17 00:01
PMCR- 2000/03/01
CRDT- 1999/09/17 00:00
PHST- 1999/09/17 00:00 [pubmed]
PHST- 1999/09/17 00:01 [medline]
PHST- 1999/09/17 00:00 [entrez]
PHST- 2000/03/01 00:00 [pmc-release]
AID - S0002-9440(10)65181-4 [pii]
AID - 1866 [pii]
AID - 10.1016/S0002-9440(10)65181-4 [doi]
PST - ppublish
SO  - Am J Pathol. 1999 Sep;155(3):823-9. doi: 10.1016/S0002-9440(10)65181-4.