PMID- 10488893
OWN - NLM
STAT- MEDLINE
DCOM- 19991021
LR  - 20060612
IS  - 1027-3719 (Print)
IS  - 1027-3719 (Linking)
VI  - 3
IP  - 9
DP  - 1999 Sep
TI  - Direct detection and identification of Mycobacterium tuberculosis in 
      smear-positive sputum samples by fluorescence in situ hybridization (FISH) using 
      peptide nucleic acid (PNA) probes.
PG  - 830-7
AB  - SETTING: Peptidenucleic acid (PNA) probesdesigned for specific detection of 
      mycobacteria of the Mycobacterium tuberculosis complex (MTC) and other 
      non-tuberculous mycobacterium species (NTM) are shown to be able to penetrate the 
      mycobacterial cell wall and subsequently hybridize in situ to complementary rRNA. 
      OBJECTIVE: To demonstrate the use of fluorescein-labelled PNA probes for 
      detection and identification of M. tuberculosis in smear-positive sputum samples. 
      DESIGN: The sensitivity and specificity of the PNA probes were investigated by 
      fluorescence in situ hybridization (FISH) using cultures of mycobacterium strains 
      representing species of the MTC and NTM, respectively. RESULTS: M. tuberculosis 
      strains were detected by FISH using specific fluorescein-labelled PNA probes 
      directly in smear-positive sputum samples without changing the morphology of the 
      cells. CONCLUSION: PNA probes allow for rapid diagnosis of tuberculosis in 
      smear-positive cases.
FAU - Stender, H
AU  - Stender H
AD  - DAKO A/S, Glostrup, Denmark.
FAU - Mollerup, T A
AU  - Mollerup TA
FAU - Lund, K
AU  - Lund K
FAU - Petersen, K H
AU  - Petersen KH
FAU - Hongmanee, P
AU  - Hongmanee P
FAU - Godtfredsen, S E
AU  - Godtfredsen SE
LA  - eng
PT  - Journal Article
PL  - France
TA  - Int J Tuberc Lung Dis
JT  - The international journal of tuberculosis and lung disease : the official journal 
      of the International Union against Tuberculosis and Lung Disease
JID - 9706389
RN  - 0 (Nucleic Acid Probes)
RN  - 0 (Peptide Nucleic Acids)
SB  - IM
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Mycobacterium tuberculosis/*isolation & purification
MH  - *Nucleic Acid Probes
MH  - *Peptide Nucleic Acids
MH  - Sensitivity and Specificity
MH  - Sputum/*microbiology
EDAT- 1999/09/17 00:00
MHDA- 1999/09/17 00:01
CRDT- 1999/09/17 00:00
PHST- 1999/09/17 00:00 [pubmed]
PHST- 1999/09/17 00:01 [medline]
PHST- 1999/09/17 00:00 [entrez]
PST - ppublish
SO  - Int J Tuberc Lung Dis. 1999 Sep;3(9):830-7.