PMID- 10490319
OWN - NLM
STAT- MEDLINE
DCOM- 19990914
LR  - 20190729
IS  - 1060-3271 (Print)
IS  - 1060-3271 (Linking)
VI  - 82
IP  - 4
DP  - 1999 Jul-Aug
TI  - Glycoalkaloid concentration in Alberta potatoes determined by matrix-assisted 
      laser desorption/ionization mass spectrometry.
PG  - 908-14
AB  - Matrix-assisted laser desorption and ionization (MALDI) mass spectrometry was 
      used to determine concentrations of individual potato glycoalkaloids in tubers. 
      Samples were extracted with methanol-water and deposited on 
      2,4,6-trihydroxyacetophenone crystals. Positive ions were analyzed with a MALDI 
      time-of-flight mass spectrometer equipped with a 337 nm laser. Analyte ion 
      intensities relative to an internal standard were used to determine chaconine and 
      solanine concentrations. Calibration curves were prepared by standard additions 
      to potato tuber material. The relative standard deviations (RSDs) of triplicate 
      measurements ranged from 1 to 16%, with an average of 9%. The day-to-day RSD for 
      replicate determinations was 11%. Recoveries of analyst-prepared spikes (50 
      micrograms/g) averaged 104% for chaconine (RSD, 8%) and 98% for solanine (RSD, 
      4%). The method limit of detection was estimated to be 2 micrograms/g.
FAU - Driedger, D R
AU  - Driedger DR
AD  - University of Alberta, Department of Agriculture, Food, and Nutritional Science, 
      Edmonton, Canada.
FAU - Sporns, P
AU  - Sporns P
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J AOAC Int
JT  - Journal of AOAC International
JID - 9215446
RN  - 0 (Solanaceous Alkaloids)
RN  - 20562-02-1 (Solanine)
SB  - IM
MH  - Calibration
MH  - Reproducibility of Results
MH  - Sensitivity and Specificity
MH  - Solanaceous Alkaloids/*analysis
MH  - Solanine/*analogs & derivatives/*analysis
MH  - Solanum tuberosum/*chemistry
MH  - *Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
EDAT- 1999/09/18 00:00
MHDA- 1999/09/18 00:01
CRDT- 1999/09/18 00:00
PHST- 1999/09/18 00:00 [pubmed]
PHST- 1999/09/18 00:01 [medline]
PHST- 1999/09/18 00:00 [entrez]
PST - ppublish
SO  - J AOAC Int. 1999 Jul-Aug;82(4):908-14.