PMID- 10492005
OWN - NLM
STAT- MEDLINE
DCOM- 19991019
LR  - 20181113
IS  - 1072-0502 (Print)
IS  - 1072-0502 (Linking)
VI  - 6
IP  - 3
DP  - 1999 May-Jun
TI  - Synaptic reliability correlates with reduced susceptibility to synaptic 
      potentiation by brain-derived neurotrophic factor.
PG  - 232-42
AB  - Recent studies have implicated brain-derived neurotrophic factor (BDNF) in 
      use-dependent modification of hippocampal synapses. BDNF can rapidly potentiate 
      synaptic transmission at glutamatergic synapses by enhancing transmitter release. 
      Using simultaneous perforated patch recording from pairs and triplets of 
      glutamatergic hippocampal neurons, we have examined how the initial state of the 
      glutamatergic synapse determines its susceptibility to synaptic modification by 
      BDNF. We found that the degree of synaptic potentiation by BDNF depends on the 
      initial reliability and strength of the synapse: Relatively weak connections were 
      strongly potentiated, whereas the effect was markedly reduced at stronger 
      synapses. The degree of BDNF-induced potentiation strongly correlated with the 
      initial coefficient of variation (CV) of the amplitude of excitatory postsynaptic 
      currents (EPSCs) and inversely correlated with the initial paired-pulse 
      facilitation, suggesting that synapses with lower release probability (Pr) are 
      more susceptible to the action of BDNF. To determine whether saturation of Pr 
      could have masked the potentiation effect of BDNF in the stronger synapses, we 
      lowered the initial Pr either by reducing the extracellular Ca2+ concentration 
      ([Ca2+]o) or by bath application of adenosine. Synapses that were initially 
      strong remained unaffected by BDNF under these conditions of reduced Pr. Thus, 
      the lack of BDNF effect on synaptic efficacy cannot simply be accounted for by 
      saturation of Pr, but rather may be due to intrinsic changes associated with 
      synaptic maturation that might covary with Pr. Finally, the dependence on initial 
      synaptic strength was also found for divergent outputs of the same presynaptic 
      neuron, suggesting that synaptic terminals with different degrees of 
      responsiveness to BDNF can coexist within in the same neuron.
FAU - Berninger, B
AU  - Berninger B
AD  - Department of Biology, University of California San Diego, La Jolla 92093-0357, 
      USA.
FAU - Schinder, A F
AU  - Schinder AF
FAU - Poo, M M
AU  - Poo MM
LA  - eng
GR  - NS37831/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Learn Mem
JT  - Learning & memory (Cold Spring Harbor, N.Y.)
JID - 9435678
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Recombinant Proteins)
RN  - 3KX376GY7L (Glutamic Acid)
RN  - 56-12-2 (gamma-Aminobutyric Acid)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/*pharmacology
MH  - Cells, Cultured
MH  - Excitatory Postsynaptic Potentials/drug effects/physiology
MH  - Glutamic Acid/physiology
MH  - Hippocampus/cytology/drug effects
MH  - Humans
MH  - Membrane Potentials/drug effects/physiology
MH  - Neuronal Plasticity/drug effects/*physiology
MH  - Patch-Clamp Techniques
MH  - Rats
MH  - Recombinant Proteins/pharmacology
MH  - Synapses/*drug effects/*physiology
MH  - Synaptic Transmission/drug effects/*physiology
MH  - gamma-Aminobutyric Acid/physiology
PMC - PMC311306
EDAT- 1999/09/24 00:00
MHDA- 1999/09/24 00:01
PMCR- 2000/05/01
CRDT- 1999/09/24 00:00
PHST- 1999/09/24 00:00 [pubmed]
PHST- 1999/09/24 00:01 [medline]
PHST- 1999/09/24 00:00 [entrez]
PHST- 2000/05/01 00:00 [pmc-release]
PST - ppublish
SO  - Learn Mem. 1999 May-Jun;6(3):232-42.