PMID- 10493730 OWN - NLM STAT- MEDLINE DCOM- 19991014 LR - 20191023 IS - 1529-2401 (Electronic) IS - 0270-6474 (Print) IS - 0270-6474 (Linking) VI - 19 IP - 19 DP - 1999 Oct 1 TI - Differential roles of Ca(2+)/calmodulin-dependent protein kinase II and mitogen-activated protein kinase activation in hippocampal long-term potentiation. PG - 8292-9 AB - The roles of Ca(2+)/calmodulin-dependent protein kinase II (CaM kinase II) and mitogen-activated protein kinase (MAPK) in long-term potentiation (LTP) were investigated in the CA1 area of hippocampal slices, using electrophysiological and biochemical approaches. A brief high-frequency stimulation, but not low-frequency stimulation, delivered to Schaffer collateral/commissural afferents produced a stable LTP and activated both CaM kinase II and 42 kDa MAPK. Different from the activity of CaM kinase II, the increase in MAPK activity was transient. At a concentration of 50 microM, but not of 30 microM, PD098059, a potent inhibitor of MAPK kinase, markedly inhibited the induction of LTP. Although the two concentrations had similar inhibitory effects on MAPK activity, only 50 microM PD098059 suppressed the activation of CaM kinase II. Application of calmidazolium, an antagonist of calmodulin, blocked both CaM kinase II activation and the LTP induction without affecting the increase in 42 kDa MAPK activity. Application of neurotrophin brain-derived neurotrophic factor (BDNF) promoted the induction of LTP, with concomitant activation of CaM kinase II. Under the same conditions, BDNF failed to activate MAPK in hippocampal slices. These results indicate that, although the LTP induction is accompanied by increases in two kinase activities, only CaM kinase II activation is required for this event. FAU - Liu, J AU - Liu J AD - Department of Pharmacology, Kumamoto University School of Medicine, Kumamoto 860-0811, Japan. FAU - Fukunaga, K AU - Fukunaga K FAU - Yamamoto, H AU - Yamamoto H FAU - Nishi, K AU - Nishi K FAU - Miyamoto, E AU - Miyamoto E LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - United States TA - J Neurosci JT - The Journal of neuroscience : the official journal of the Society for Neuroscience JID - 8102140 RN - 0 (Brain-Derived Neurotrophic Factor) RN - 0 (Enzyme Inhibitors) RN - 0 (Flavonoids) RN - 0 (Isoenzymes) RN - EC 2.7.11.17 (Calcium-Calmodulin-Dependent Protein Kinase Type 2) RN - EC 2.7.11.17 (Calcium-Calmodulin-Dependent Protein Kinases) RN - SJE1IO5E3I (2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one) SB - IM MH - Animals MH - Brain-Derived Neurotrophic Factor/pharmacology MH - Calcium-Calmodulin-Dependent Protein Kinase Type 2 MH - Calcium-Calmodulin-Dependent Protein Kinases/isolation & purification/*metabolism MH - Electric Stimulation MH - Electrophoresis, Polyacrylamide Gel MH - Enzyme Activation MH - Enzyme Inhibitors/pharmacology MH - Flavonoids/pharmacology MH - Hippocampus/*physiology MH - In Vitro Techniques MH - Isoenzymes/isolation & purification/metabolism MH - Kinetics MH - Long-Term Potentiation/drug effects/*physiology MH - Male MH - Phosphorylation MH - Rats MH - Rats, Sprague-Dawley PMC - PMC6783055 EDAT- 1999/09/24 00:00 MHDA- 1999/09/24 00:01 PMCR- 2000/04/01 CRDT- 1999/09/24 00:00 PHST- 1999/09/24 00:00 [pubmed] PHST- 1999/09/24 00:01 [medline] PHST- 1999/09/24 00:00 [entrez] PHST- 2000/04/01 00:00 [pmc-release] AID - 3477 [pii] AID - 10.1523/JNEUROSCI.19-19-08292.1999 [doi] PST - ppublish SO - J Neurosci. 1999 Oct 1;19(19):8292-9. doi: 10.1523/JNEUROSCI.19-19-08292.1999.