PMID- 10494920
OWN - NLM
STAT- MEDLINE
DCOM- 19990927
LR  - 20190513
IS  - 0368-2811 (Print)
IS  - 0368-2811 (Linking)
VI  - 29
IP  - 8
DP  - 1999 Aug
TI  - Analysis of myelodysplastic syndrome clones arising after multiple myeloma: a 
      case study by correlative interphase cytogenetic analysis.
PG  - 374-7
AB  - BACKGROUND: A patient with multiple myeloma developed myelodysplastic syndrome 
      (MDS). Chromosomal analysis performed after the development of MDS revealed 
      monosomy of chromosome 9 in all the meta-phases. We wished to identify the extent 
      of the clone with the chromosomal abnormality originating from MDS clone. 
      METHODS: A correlative interphase study by fluorescence in situ hybridization 
      (FISH) was performed and we determined whether each lineage of cells obtained the 
      molecular mark. The chromosome 9 classic alpha satellite region DNA was used as a 
      probe for the FISH analysis in smear specimens stained with Wright-Giemsa stain. 
      RESULTS: Erythroblasts, granulocytes and myelocytes had only one signal, whereas 
      myeloma cells showed two to four signals. CONCLUSION: This study visualized the 
      spectrum of MDS clone. The results suggest that the origin of MDS is different 
      from that of multiple myeloma, at least in this case.
FAU - Kobayashi, Y
AU  - Kobayashi Y
AD  - Department of Hematology/Oncology, Institute of Medical Science, The University 
      of Tokyo, Japan. ykkobaya@gan2.ncc.go.jp
FAU - Nakayama, M
AU  - Nakayama M
FAU - Uemura, N
AU  - Uemura N
FAU - Takeyama, K
AU  - Takeyama K
FAU - Tobinai, K
AU  - Tobinai K
FAU - Takenaka, T
AU  - Takenaka T
FAU - Choi, S H
AU  - Choi SH
FAU - Satoh, H
AU  - Satoh H
FAU - Mori, S
AU  - Mori S
FAU - Asano, S
AU  - Asano S
LA  - eng
PT  - Case Reports
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Jpn J Clin Oncol
JT  - Japanese journal of clinical oncology
JID - 0313225
SB  - IM
MH  - *Chromosomes, Human, Pair 9
MH  - Clone Cells
MH  - Cytogenetics
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Interphase
MH  - Middle Aged
MH  - *Monosomy
MH  - Multiple Myeloma/*complications/pathology
MH  - Myelodysplastic Syndromes/etiology/*genetics
EDAT- 1999/09/24 00:00
MHDA- 1999/09/24 00:01
CRDT- 1999/09/24 00:00
PHST- 1999/09/24 00:00 [pubmed]
PHST- 1999/09/24 00:01 [medline]
PHST- 1999/09/24 00:00 [entrez]
AID - 10.1093/jjco/29.8.374 [doi]
PST - ppublish
SO  - Jpn J Clin Oncol. 1999 Aug;29(8):374-7. doi: 10.1093/jjco/29.8.374.