PMID- 10497014
OWN - NLM
STAT- MEDLINE
DCOM- 19991105
LR  - 20061115
IS  - 1521-6616 (Print)
IS  - 1521-6616 (Linking)
VI  - 93
IP  - 1
DP  - 1999 Oct
TI  - Antibodies against Trypanosoma cruzi alkaline antigens are elicited in sera from 
      acute but not chronic human chagasic patients.
PG  - 81-9
AB  - The aim of this work was to study the antibody response of acute and chronic 
      chagasic patients against a Trypanosoma cruzi alkaline fraction (FI) in 
      comparison with the reactivity against a T. cruzi acidic antigen, the main 
      cystein proteinase of the parasite named cruzipain, and "natural" antigens. 
      FI-specific antibodies were detected only during the acute phase of the infection 
      and IgM was the main isotype produced, whereas cruzipain-specific antibodies were 
      detected during all phases of the infection. By means of immunoblot and 
      sequencing analysis we identified a 47-kDa FI proteic band recognized by IgM from 
      acute chagasic patients as the T. cruzi glutamate dehydrogenase (GluDH). 
      Furthermore, the antibody response against isolated GluDH showed similar 
      characteristics as the one against FI. We also observed a strict association 
      between the reactivity of IgM against FI and GluDH and IgM natural antibodies. 
      However, reactivity against these alkaline antigens was not modified after 
      absorption of natural antibodies in sera from acute chagasic patients, indicating 
      that these parasite antigens are not recognized by the polyspecific natural 
      antibodies. The most important goal of this report is that for the first time the 
      T. cruzi antigen isoelectric point has been associated with its ability to 
      trigger immunological memory, raising a novel antigen property that should be 
      considered in the selection of antigens used in Chagas' disease diagnostic test 
      and in the design of a vaccine against T. cruzi infection.
CI  - Copyright 1999 Academic Press.
FAU - Zuniga, E
AU  - Zuniga E
AD  - Departamento de Bioquimica Clinica, Facultad de Ciencias Quimicas (UNC), Cordoba, 
      5000, Argentina.
FAU - Montes, C
AU  - Montes C
FAU - Barbieri, G
AU  - Barbieri G
FAU - Gruppi, A
AU  - Gruppi A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Clin Immunol
JT  - Clinical immunology (Orlando, Fla.)
JID - 100883537
RN  - 0 (Antibodies, Protozoan)
RN  - 0 (Antigens, Protozoan)
RN  - 0 (Autoantibodies)
RN  - 0 (Immunoglobulin M)
SB  - IM
MH  - Acute Disease
MH  - Amino Acid Sequence
MH  - Animals
MH  - Antibodies, Protozoan/*blood
MH  - Antigens, Protozoan/blood
MH  - Autoantibodies/blood
MH  - Chagas Disease/*blood
MH  - Child
MH  - Child, Preschool
MH  - Chronic Disease
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Humans
MH  - Immunoglobulin M/immunology
MH  - Infant
MH  - Trypanosoma cruzi/*immunology
EDAT- 1999/09/25 00:00
MHDA- 1999/09/25 00:01
CRDT- 1999/09/25 00:00
PHST- 1999/09/25 00:00 [pubmed]
PHST- 1999/09/25 00:01 [medline]
PHST- 1999/09/25 00:00 [entrez]
AID - S1521-6616(99)94744-7 [pii]
AID - 10.1006/clim.1999.4744 [doi]
PST - ppublish
SO  - Clin Immunol. 1999 Oct;93(1):81-9. doi: 10.1006/clim.1999.4744.