PMID- 10497311
OWN - NLM
STAT- MEDLINE
DCOM- 19991101
LR  - 20121115
IS  - 0021-9541 (Print)
IS  - 0021-9541 (Linking)
VI  - 181
IP  - 2
DP  - 1999 Nov
TI  - Hepatocyte growth factor/scatter factor decreases the expression of occludin and 
      transendothelial resistance (TER) and increases paracellular permeability in 
      human vascular endothelial cells.
PG  - 319-29
AB  - Hepatocyte growth factor/scatter factor (HGF/SF), a multi function cytokine has 
      been shown to regulate the expression of cell adhesion molecules in endothelial 
      cells. In the current study, we examined the effects of HGF/SF on the function of 
      tight junctions and the expression of occludin in these cells. Stimulation of 
      human vascular endothelial cells with HGF/SF resulted in a concentration 
      dependent increase in the paracellular permeability as measured using 
      fluorescence labelled dextran but a decrease in the transendothelial cell 
      resistance (TER) of the endothelial cells. Western blotting revealed that HGF/SF 
      decreased the level of occludin in the cells, a primary tight junction forming 
      protein. Immunofluorescence study indicated that shortly after treatment with 
      HGF/SF there was a disturbance of the distribution of occludin and then a 
      reduction in the staining of the molecule. It is concluded that HGF/SF decreases 
      the expression of occludin, resulting in the functional change of tight junction.
CI  - Copyright 1999 Wiley-Liss, Inc.
FAU - Jiang, W G
AU  - Jiang WG
AD  - Metastasis Research Group, University Department of Surgery, University of Wales 
      College of Medicine, Cardiff, United Kingdom. jiangw@cf.ac.uk
FAU - Martin, T A
AU  - Martin TA
FAU - Matsumoto, K
AU  - Matsumoto K
FAU - Nakamura, T
AU  - Nakamura T
FAU - Mansel, R E
AU  - Mansel RE
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Cell Physiol
JT  - Journal of cellular physiology
JID - 0050222
RN  - 0 (Membrane Proteins)
RN  - 0 (OCLN protein, human)
RN  - 0 (Occludin)
RN  - 0 (Phosphoproteins)
RN  - 0 (TJP1 protein, human)
RN  - 0 (Zonula Occludens-1 Protein)
RN  - 67256-21-7 (Hepatocyte Growth Factor)
SB  - IM
MH  - Cell Line
MH  - Cell Membrane Permeability/*drug effects/physiology
MH  - Endothelium, Vascular/drug effects/*physiology
MH  - Gene Expression Regulation/drug effects/*physiology
MH  - Hepatocyte Growth Factor/*pharmacology
MH  - Humans
MH  - Kinetics
MH  - Membrane Proteins/analysis/*genetics
MH  - Occludin
MH  - Phosphoproteins/analysis/genetics
MH  - Tight Junctions/drug effects/*physiology
MH  - Zonula Occludens-1 Protein
EDAT- 1999/09/25 00:00
MHDA- 1999/09/25 00:01
CRDT- 1999/09/25 00:00
PHST- 1999/09/25 00:00 [pubmed]
PHST- 1999/09/25 00:01 [medline]
PHST- 1999/09/25 00:00 [entrez]
AID - 10.1002/(SICI)1097-4652(199911)181:2<319::AID-JCP14>3.0.CO;2-S [pii]
AID - 10.1002/(SICI)1097-4652(199911)181:2<319::AID-JCP14>3.0.CO;2-S [doi]
PST - ppublish
SO  - J Cell Physiol. 1999 Nov;181(2):319-29. doi: 
      10.1002/(SICI)1097-4652(199911)181:2<319::AID-JCP14>3.0.CO;2-S.