PMID- 10497876
OWN - NLM
STAT- MEDLINE
DCOM- 19991124
LR  - 20190818
IS  - 0300-8177 (Print)
IS  - 0300-8177 (Linking)
VI  - 198
IP  - 1-2
DP  - 1999 Aug
TI  - Modulation of Ca2+ mobilization by protein kinase C in the submandibular duct 
      cell line A253.
PG  - 39-46
AB  - The expression of protein kinase C (PKC) isoforms and the modulation of Ca2+ 
      mobilization by PKC were investigated in the human submandibular duct cell line 
      A253. Three new PKC (nPKC) isoforms (delta, epsilon, and theta) and one atypical 
      PKC (aPKC) isoform (lambda) are expressed in this cell line. No classical PKC 
      (cPKC) isoforms were present. The effects of the PKC activator phorbol 
      12-myristate-13-acetate (PMA) and of the PKC inhibitors calphostin C (CC) and 
      bisindolymaleimide I (BSM) on inositol 1,4,5-trisphosphate (IP3) and Ca2+ 
      responses to ATP and to thapsigargin (TG) were investigated. Pre-exposure to PMA 
      inhibited IP3 formation, Ca2+ release and Ca2+ influx in response to ATP. 
      Pre-exposure to CC or BSM slightly enhanced IP3 formation but inhibited the Ca2+ 
      release and the Ca2+ influx induced by ATP. In contrast, pre-exposure to PMA did 
      not modify the Ca2+ release induced by TG, but reduced the influx of Ca2+ seen in 
      the presence of this Ca2+-ATPase inhibitor. These results suggest that PKC 
      modulates elements of the IP3/Ca2+ signal transduction pathway in A253 cells by 
      (1) inhibiting phosphatidylinositol turnover and altering the sensitivity of the 
      Ca2+ channels to IP3, (2) altering the activity, the sensitivity to inhibitors, 
      or the distribution of the TG-sensitive Ca2+ ATPase, and (3) modulating Ca2+ 
      entry pathways.
FAU - Sugita, K
AU  - Sugita K
AD  - Department of Pediatrics, University of Texas Health Science Center, San Antonio 
      78284-7827, USA.
FAU - Mork, A C
AU  - Mork AC
FAU - Zhang, G H
AU  - Zhang GH
FAU - Martinez, J R
AU  - Martinez JR
LA  - eng
GR  - DE09270/DE/NIDCR NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Netherlands
TA  - Mol Cell Biochem
JT  - Molecular and cellular biochemistry
JID - 0364456
RN  - 0 (Isoenzymes)
RN  - 0 (Phosphatidylinositols)
RN  - 67526-95-8 (Thapsigargin)
RN  - EC 2.7.11.13 (Protein Kinase C)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Calcium/*metabolism
MH  - Cell Line
MH  - Enzyme Activation
MH  - Humans
MH  - Isoenzymes/antagonists & inhibitors/*metabolism
MH  - Phosphatidylinositols/biosynthesis/metabolism
MH  - Protein Kinase C/antagonists & inhibitors/*metabolism
MH  - Salivary Ducts/cytology/enzymology/*metabolism
MH  - Signal Transduction
MH  - Submandibular Gland/cytology/enzymology/*metabolism
MH  - Thapsigargin/pharmacology
EDAT- 1999/09/25 00:00
MHDA- 1999/09/25 00:01
CRDT- 1999/09/25 00:00
PHST- 1999/09/25 00:00 [pubmed]
PHST- 1999/09/25 00:01 [medline]
PHST- 1999/09/25 00:00 [entrez]
AID - 10.1023/a:1006925408055 [doi]
PST - ppublish
SO  - Mol Cell Biochem. 1999 Aug;198(1-2):39-46. doi: 10.1023/a:1006925408055.