PMID- 10498822
OWN - NLM
STAT- MEDLINE
DCOM- 19991122
LR  - 20191210
IS  - 0894-1491 (Print)
IS  - 0894-1491 (Linking)
VI  - 28
IP  - 1
DP  - 1999 Oct
TI  - Thapsigargin induces microglial transformation from amoeboid to ramified type in 
      vitro.
PG  - 49-52
AB  - Microglia generally display amoeboid morphology under prevalent culture 
      conditions. We found that cultured microglia derived from rat cerebral cortex 
      undergo a morphological transformation from amoeboid to process-bearing microglia 
      upon treatment with thapsigargin (TG), a specific Ca2+-ATPase inhibitor of 
      endoplasmic reticulum. Microglial transformation was further enhanced by exposure 
      of amoeboid microglia to serum-free (N2) medium containing TG (TG/N2 treatment). 
      TG/N2-treated microglia showed a marked reduction in the activity of phagocytosis 
      and showed down-regulated expression of MRF-1 or F4/80, which are markers for 
      activated microglia. Thus, both morphological and physiological criteria suggest 
      that TG promotes the ramification of amoeboid microglia in vitro. This method 
      would be helpful in characterization of ramified microglia in vitro.
CI  - Copyright 1999 Wiley-Liss, Inc.
FAU - Yagi, R
AU  - Yagi R
AD  - Molecular Neurobiology Laboratory, Graduate School of Science, Hokkaido 
      University, Sapporo, Japan.
FAU - Tanaka, S
AU  - Tanaka S
FAU - Koike, T
AU  - Koike T
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Glia
JT  - Glia
JID - 8806785
RN  - 0 (Culture Media, Serum-Free)
RN  - 0 (Enzyme Inhibitors)
RN  - 67526-95-8 (Thapsigargin)
RN  - EC 7.2.2.10 (Calcium-Transporting ATPases)
SB  - IM
EIN - Glia 2000 Jan 1;29(1):102
MH  - Animals
MH  - Calcium-Transporting ATPases/*antagonists & inhibitors
MH  - Cell Polarity/drug effects
MH  - Cells, Cultured
MH  - Cerebral Cortex/cytology/drug effects/enzymology
MH  - Culture Media, Serum-Free
MH  - Enzyme Inhibitors/*pharmacology
MH  - Microglia/*drug effects/enzymology/*ultrastructure
MH  - Phagocytosis/drug effects
MH  - Rats
MH  - Thapsigargin/*pharmacology
EDAT- 1999/09/28 09:00
MHDA- 2000/03/04 09:00
CRDT- 1999/09/28 09:00
PHST- 1999/09/28 09:00 [pubmed]
PHST- 2000/03/04 09:00 [medline]
PHST- 1999/09/28 09:00 [entrez]
AID - 10.1002/(SICI)1098-1136(199910)28:1<49::AID-GLIA6>3.0.CO;2-V [pii]
AID - 10.1002/(sici)1098-1136(199910)28:1<49::aid-glia6>3.0.co;2-v [doi]
PST - ppublish
SO  - Glia. 1999 Oct;28(1):49-52. doi: 
      10.1002/(sici)1098-1136(199910)28:1<49::aid-glia6>3.0.co;2-v.