PMID- 10499442
OWN - NLM
STAT- MEDLINE
DCOM- 19991007
LR  - 20190513
IS  - 0022-3069 (Print)
IS  - 0022-3069 (Linking)
VI  - 58
IP  - 9
DP  - 1999 Sep
TI  - PrPc glycoform heterogeneity as a function of brain region: implications for 
      selective targeting of neurons by prion strains.
PG  - 1000-9
AB  - We recently found that deletion of the Asn-linked carbohydrate (CHO) at residue 
      197 of Syrian hamster (SHa) PrP(C) while retaining the CHO at Asn 181 has a 
      profound effect on which population of neurons are targeted for conversion of 
      SHaPrP(C) to SHaPrP(Sc) in transgenic (Tg) mice inoculated with scrapie prions. 
      We hypothesized that selective targeting of neuronal populations is determined by 
      cell-specific differences in the affinity of an infecting PrP(Sc) (prion) for 
      PrP(C) and that the affinity might be modulated by nerve cell-specific 
      differences in PrP(C) glycosylation. Here we tested this hypothesis by assessing 
      whether or not each brain region in Syrian hamsters synthesizes different PrP(C) 
      glycoforms, as inferred from 2D-gel electrophoresis. Reproducible differences in 
      the number and isoelectric point of PrP(C) charge isomers were found as a 
      function of brain region. The results of this study support the hypothesis that 
      the PrP(Sc) accumulation and the vacuolation pattern phenotypes in the brain are 
      governed by neuron-specific differences in PrP(C) glycoforms.
FAU - DeArmond, S J
AU  - DeArmond SJ
AD  - Department of Pathology, University of California, San Francisco 94143-0506, USA.
FAU - Qiu, Y
AU  - Qiu Y
FAU - Sanchez, H
AU  - Sanchez H
FAU - Spilman, P R
AU  - Spilman PR
FAU - Ninchak-Casey, A
AU  - Ninchak-Casey A
FAU - Alonso, D
AU  - Alonso D
FAU - Daggett, V
AU  - Daggett V
LA  - eng
GR  - AG02132/AG/NIA NIH HHS/United States
GR  - AG10770/AG/NIA NIH HHS/United States
GR  - NS14069/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - J Neuropathol Exp Neurol
JT  - Journal of neuropathology and experimental neurology
JID - 2985192R
RN  - 0 (PrPC Proteins)
RN  - 0 (Prions)
RN  - 0 (Protein Isoforms)
SB  - IM
MH  - Animals
MH  - Brain/*metabolism
MH  - Cricetinae
MH  - Electrophoresis, Gel, Two-Dimensional
MH  - Isoelectric Point
MH  - Mesocricetus
MH  - Neurons/*virology
MH  - Osmolar Concentration
MH  - PrPC Proteins/chemistry/*metabolism
MH  - Prions/genetics/*physiology
MH  - Protein Isoforms/chemistry/metabolism
MH  - Tissue Distribution
EDAT- 1999/09/28 00:00
MHDA- 1999/09/28 00:01
CRDT- 1999/09/28 00:00
PHST- 1999/09/28 00:00 [pubmed]
PHST- 1999/09/28 00:01 [medline]
PHST- 1999/09/28 00:00 [entrez]
AID - 10.1097/00005072-199909000-00010 [doi]
PST - ppublish
SO  - J Neuropathol Exp Neurol. 1999 Sep;58(9):1000-9. doi: 
      10.1097/00005072-199909000-00010.