PMID- 10499452
OWN - NLM
STAT- MEDLINE
DCOM- 19991020
LR  - 20191210
IS  - 0920-8569 (Print)
IS  - 0920-8569 (Linking)
VI  - 19
IP  - 1
DP  - 1999
TI  - The characterization and phylogenetic relationship of the Trichoplusia ni single 
      capsid nuclear polyhedrosis virus polyhedrin gene.
PG  - 67-72
AB  - The polyhedrin gene (polh) was identified from the Trichoplusia ni (Tni) single 
      capsid nuclear polyhedrosis virus (SNPV). An EcoRI fragment containing the 
      truncated polyhedrin gene was detected by hybridization with an AcMNPV expression 
      vector probe; the remaining portion of the gene was amplified by reverse PCR. An 
      open reading frame (ORF) of 741 nucleotides (nt), encoding a putative protein of 
      246 amino acids (a.a) with Mr 28,780 Da was identified. The 5'-noncoding region 
      contained the putative late (TAAG) transcription initiation motif. The 3' end, 
      downstream of the translation stop codon, lacked an obvious putative poly (A) 
      signal. Nucleotide and amino acid homology are greater than 80% to that of 
      Mamestra brassicae polyhedrin sequences. Results suggest that T. niSNPV is a 
      member of the group II nuclear polyhedrosis viruses.
FAU - Fielding, B C
AU  - Fielding BC
AD  - University of the Western Cape, Faculty of Science, Department of Microbiology, 
      Bellville, South Africa. bertie@mbiol.uwc.ac.za
FAU - Davison, S
AU  - Davison S
LA  - eng
SI  - GENBANK/AF093405
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Virus Genes
JT  - Virus genes
JID - 8803967
RN  - 0 (Occlusion Body Matrix Proteins)
RN  - 0 (Viral Proteins)
RN  - 0 (Viral Structural Proteins)
RN  - 0 (polyhedrin protein, Nucleopolyhedrovirus)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Capsid/*genetics
MH  - Lepidoptera/*virology
MH  - Molecular Sequence Data
MH  - Nucleopolyhedroviruses/*genetics
MH  - Occlusion Body Matrix Proteins
MH  - Phylogeny
MH  - Sequence Alignment
MH  - Viral Proteins/chemistry/*genetics
MH  - Viral Structural Proteins
EDAT- 1999/09/28 00:00
MHDA- 1999/09/28 00:01
CRDT- 1999/09/28 00:00
PHST- 1999/09/28 00:00 [pubmed]
PHST- 1999/09/28 00:01 [medline]
PHST- 1999/09/28 00:00 [entrez]
AID - 10.1023/a:1008192723971 [doi]
PST - ppublish
SO  - Virus Genes. 1999;19(1):67-72. doi: 10.1023/a:1008192723971.