PMID- 10499923
OWN - NLM
STAT- MEDLINE
DCOM- 19991019
LR  - 20211203
IS  - 0022-1007 (Print)
IS  - 1540-9538 (Electronic)
IS  - 0022-1007 (Linking)
VI  - 190
IP  - 6
DP  - 1999 Sep 20
TI  - A member of the dendritic cell family that enters B cell follicles and stimulates 
      primary antibody responses identified by a mannose receptor fusion protein.
PG  - 851-60
AB  - Dendritic cells (DCs) are known to activate naive T cells to become effective 
      helper cells. In addition, recent evidence suggests that DCs may influence naive 
      B cells during the initial priming of antibody responses. In this study, using 
      three-color confocal microscopy and three-dimensional immunohistograms, we have 
      observed that in the first few days after a primary immunization, cells with 
      dendritic morphology progressively localize within primary B cell follicles. 
      These cells were identified by their ability to bind a fusion protein consisting 
      of the terminal cysteine-rich portion of the mouse mannose receptor and the Fc 
      portion of human immunoglobulin (Ig)G1 (CR-Fc). In situ, these CR-Fc binding 
      cells express major histocompatibility complex class II, sialoadhesin, and CD11c 
      and are negative for other markers identifying the myeloid DC lineage, such as 
      (CD11b), macrophages (F4/80), follicular DCs (FDC-M2), B cells (B220), and T 
      cells (CD4). Using CR-Fc binding capacity and flow cytometry, the cells were 
      purified from the draining lymph nodes of mice 24 h after immunization. When 
      injected into naive mice, these cells were able to prime T cells as well as 
      induce production of antigen-specific IgM and IgG1. Furthermore, they produced 
      significantly more of the lymphocyte chemoattractant, macrophage inflammatory 
      protein (MIP)-1alpha, than isolated interdigitating cells. Taken together, these 
      results provide evidence that a subset of DCs enters primary follicles, armed 
      with the capacity to attract and provide antigenic stimulation for T and B 
      lymphocytes.
FAU - Berney, C
AU  - Berney C
AD  - Serono Pharmaceutical Research Institute, CH-1228 Geneva, Switzerland.
FAU - Herren, S
AU  - Herren S
FAU - Power, C A
AU  - Power CA
FAU - Gordon, S
AU  - Gordon S
FAU - Martinez-Pomares, L
AU  - Martinez-Pomares L
FAU - Kosco-Vilbois, M H
AU  - Kosco-Vilbois MH
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Exp Med
JT  - The Journal of experimental medicine
JID - 2985109R
RN  - 0 (Antigens, CD)
RN  - 0 (Immunoglobulin G)
RN  - 0 (Lectins, C-Type)
RN  - 0 (Mannose Receptor)
RN  - 0 (Mannose-Binding Lectins)
RN  - 0 (Receptors, Cell Surface)
RN  - 0 (Receptors, Fc)
RN  - 0 (Recombinant Fusion Proteins)
SB  - IM
MH  - Animals
MH  - *Antigen Presentation
MH  - Antigens, CD/immunology
MH  - B-Lymphocytes/*immunology
MH  - Dendritic Cells/*immunology
MH  - Female
MH  - Humans
MH  - *Immunity
MH  - Immunoglobulin G/immunology
MH  - *Lectins, C-Type
MH  - Lymphocyte Cooperation
MH  - Mannose Receptor
MH  - *Mannose-Binding Lectins
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Receptors, Cell Surface/genetics/*immunology
MH  - Receptors, Fc/genetics/*immunology
MH  - Recombinant Fusion Proteins/genetics/immunology
MH  - T-Lymphocytes/*immunology
PMC - PMC2195630
EDAT- 1999/09/28 00:00
MHDA- 1999/09/28 00:01
PMCR- 2000/03/20
CRDT- 1999/09/28 00:00
PHST- 1999/09/28 00:00 [pubmed]
PHST- 1999/09/28 00:01 [medline]
PHST- 1999/09/28 00:00 [entrez]
PHST- 2000/03/20 00:00 [pmc-release]
AID - 99-0356 [pii]
AID - 10.1084/jem.190.6.851 [doi]
PST - ppublish
SO  - J Exp Med. 1999 Sep 20;190(6):851-60. doi: 10.1084/jem.190.6.851.