PMID- 10501624
OWN - NLM
STAT- MEDLINE
DCOM- 19991115
LR  - 20190910
IS  - 0192-0561 (Print)
IS  - 0192-0561 (Linking)
VI  - 21
IP  - 9
DP  - 1999 Sep
TI  - Dual effect of spermine on mast cell secretion exhibits different calcium and 
      temperature requirements.
PG  - 547-59
AB  - Mast cells release many biologically active molecules upon stimulation by a 
      variety of molecules such as immunoglobulin E (IgE) and specific antigen, 
      anaphylatoxins, as well as a number of cationic compounds which include drugs, 
      kinins and neuropeptides. The effect of the naturally occurring polyamine 
      spermine was studied because, even though it is polycationic, it has been 
      implicated in the modulation of secretory processes in a variety of cells. In 
      particular, it was previously shown that oxidation products of spermine inhibit 
      mast cell secretion. High concentrations of spermine (5 x 10(-3) M) added at 37 
      degrees C induced mast cell secretion that had similar characteristics with that 
      triggered by compound 48/80 (48/80). However, spermine inhibited mast cell 
      secretion in a dose-dependent manner as long as it was added at 4-10 degrees C 
      for at least 10 min in the absence of Ca++ before warming the cells to 37 degrees 
      C and triggering them with 48/80. These findings were true both for purified rat 
      peritoneal mast cells and for rat skin mast cells in situ. Addition of calcium 
      after the cells had been warmed to 37 degrees C could not reverse this 
      inhibition. The inhibition seen when spermine was added at 4 degrees C was, 
      however, overcome if phorbol myristate acetate (PMA) or NaF, which activate PKC 
      and G proteins respectively, were added to mast cells at 37 degrees C together 
      with Ca++. These results indicate that polyamines could be important modulators 
      of the activation state of mast cells and might help further define the 
      biochemical events involved in mast cell secretion.
FAU - Vliagoftis, H
AU  - Vliagoftis H
AD  - Department of Pharmacology and Experimental Therapeutics, Tufts University School 
      of Medicine, Boston, MA, USA.
FAU - Mak, L
AU  - Mak L
FAU - Boucher, W
AU  - Boucher W
FAU - Theoharides, T C
AU  - Theoharides TC
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Int J Immunopharmacol
JT  - International journal of immunopharmacology
JID - 7904799
RN  - 0 (Virulence Factors, Bordetella)
RN  - 2FZ7Y3VOQX (Spermine)
RN  - 333DO1RDJY (Serotonin)
RN  - 4091-50-3 (p-Methoxy-N-methylphenethylamine)
RN  - 9012-63-9 (Cholera Toxin)
RN  - H88EPA0A3N (Staurosporine)
RN  - NI40JAQ945 (Tetradecanoylphorbol Acetate)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Animals
MH  - Calcium/*metabolism
MH  - Cholera Toxin/pharmacology
MH  - Depression, Chemical
MH  - In Vitro Techniques
MH  - Male
MH  - Mast Cells/*drug effects/metabolism
MH  - Peritoneal Cavity/cytology
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Serotonin/metabolism
MH  - Spermine/*pharmacology
MH  - Staurosporine/pharmacology
MH  - Temperature
MH  - Tetradecanoylphorbol Acetate/pharmacology
MH  - Virulence Factors, Bordetella/pharmacology
MH  - p-Methoxy-N-methylphenethylamine/pharmacology
EDAT- 1999/09/29 00:00
MHDA- 1999/09/29 00:01
CRDT- 1999/09/29 00:00
PHST- 1999/09/29 00:00 [pubmed]
PHST- 1999/09/29 00:01 [medline]
PHST- 1999/09/29 00:00 [entrez]
AID - S0192-0561(99)00031-4 [pii]
AID - 10.1016/s0192-0561(99)00031-4 [doi]
PST - ppublish
SO  - Int J Immunopharmacol. 1999 Sep;21(9):547-59. doi: 10.1016/s0192-0561(99)00031-4.