PMID- 10501833
OWN - NLM
STAT- MEDLINE
DCOM- 19991102
LR  - 20190822
IS  - 0022-2631 (Print)
IS  - 0022-2631 (Linking)
VI  - 171
IP  - 3
DP  - 1999 Oct 1
TI  - Regulation of intracellular Ca(2+) by CFTR in Chinese hamster ovary cells.
PG  - 255-65
AB  - In cystic fibrosis, the mutation of the CFTR protein causes reduced 
      transepithelial Cl(-) secretion. As recently proposed, beside its role of Cl(-) 
      channel, CFTR may regulate the activity of other channels such as a 
      Ca(2+)-activated Cl(-) channel. Using a calcium imaging system, we show, in 
      adenovirus-CFTR infected Chinese Hamster Ovary (CHO) cell monolayers, that CFTR 
      can act as a regulator of intracellular [Ca(2+)](i) ([Ca(2+)](i)), involving 
      purino-receptors. Apical exposure to ATP or UTP produced an increase in 
      ([Ca(2+)](i) in noninfected CHO cell monolayers (CHO-WT), in CHO monolayers 
      infected with an adenovirus-CFTR (CHO-CFTR) or infected with an adenovirus-LacZ 
      (CHO-LacZ). The transient [Ca(2+)](i) increase produced by ATP or UTP could be 
      mimicked by activation of CFTR with forskolin (20 microm) in CHO-CFTR confluent 
      monolayers. However, forskolin had no significant effect on [Ca(2+)](i) in 
      noninfected CHO-WT or in CHO-LacZ cells. Pretreatment with purino-receptor 
      antagonists such as suramin (100 microm) or reactive blue-2. (100 microm), and 
      with hexokinase (0.28 U/mg) inhibited the [Ca(2+)](i) response to forskolin in 
      CHO-CFTR infected cells. Taken together, our experiments provide evidence for 
      purino-receptor activation by ATP released from the cell and regulation of 
      [Ca(2+)](i) by CFTR in CHO epithelial cell membranes.
FAU - Urbach, V
AU  - Urbach V
AD  - Wellcome Trust Cellular Physiology Research Unit, Department of Physiology, 
      University College, Cork, Ireland.
FAU - Harvey, B J
AU  - Harvey BJ
LA  - eng
GR  - Wellcome Trust/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Membr Biol
JT  - The Journal of membrane biology
JID - 0211301
RN  - 0 (Receptors, Purinergic)
RN  - 126880-72-6 (Cystic Fibrosis Transmembrane Conductance Regulator)
RN  - 1F7A44V6OU (Colforsin)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Animals
MH  - CHO Cells
MH  - Calcium/*physiology
MH  - Colforsin/pharmacology
MH  - Cricetinae
MH  - Cystic Fibrosis Transmembrane Conductance Regulator/*physiology
MH  - Ion Transport/physiology
MH  - Receptors, Purinergic/*physiology
MH  - Signal Transduction
EDAT- 1999/09/29 00:00
MHDA- 1999/09/29 00:01
CRDT- 1999/09/29 00:00
PHST- 1999/09/29 00:00 [pubmed]
PHST- 1999/09/29 00:01 [medline]
PHST- 1999/09/29 00:00 [entrez]
AID - JMEMB102 [pii]
AID - 10.1007/s002329900576 [doi]
PST - ppublish
SO  - J Membr Biol. 1999 Oct 1;171(3):255-65. doi: 10.1007/s002329900576.