PMID- 10502251
OWN - NLM
STAT- MEDLINE
DCOM- 19991018
LR  - 20190905
IS  - 0021-9967 (Print)
IS  - 0021-9967 (Linking)
VI  - 413
IP  - 3
DP  - 1999 Oct 25
TI  - Characterization of dopaminergic midbrain neurons in a DBH:BDNF transgenic mouse.
PG  - 449-62
AB  - The neurotrophin brain-derived neurotrophic factor (BDNF) has been implicated in 
      the survival and differentiation of central nervous system neurons, including 
      dopaminergic cells in culture. To determine whether BDNF might play a role in the 
      development of dopaminergic neurons in vivo, we used a previously characterized 
      transgenic mouse (DBH:BDNF) that overexpresses BDNF in adrenergic and 
      noradrenergic neurons as a result of fusion of the BDNF gene to the dopamine 
      beta-hydroxylase (DBH) gene promoter. We quantified dopaminergic neuronal 
      profiles at four midbrain coronal levels and compared DBH:BDNF transgenic animals 
      with wild-type mice of the same genetic background. Analysis of sections 
      immunostained with tyrosine hydroxylase (TH) showed that the mean number of 
      dopaminergic neurons in the four selected midbrain sections was 52% greater 
      (one-way analysis of variance, P < 0.0005) in transgenic mice (2,165 +/- 55 S. 
      E.M., n = 4) than in control mice (1,428 +/- 71 S.E.M., n = 4). The increase in 
      dopaminergic neuron profile count in DBH:BDNF transgenic animals was confirmed by 
      analysis of the pars compacta of the substantia nigra on Nissl-stained sections. 
      Surface area of the reference region of interest containing TH-immunoreactive 
      neurons was similar in transgenic and control mice. Regional analysis of 
      different midbrain areas containing dopaminergic neurons suggested that the 
      increase in cell profile count occurs in a relatively homogeneous manner. 
      Comparison of TH-immunoreactive cell size showed a tendency for smaller neurons 
      in transgenic animals, but the difference was not statistically significant. We 
      conclude that DBH:BDNF transgenic mice show increased number of TH-immunoreactive 
      cells in the midbrain. We propose that BDNF rescues dopaminergic neurons from the 
      perinatal period of developmental cell death as a consequence of increased 
      anterograde transport of the neurotrophin via the coeruleonigral projection.
CI  - Copyright 1999 Wiley-Liss, Inc.
FAU - Alonso-Vanegas, M A
AU  - Alonso-Vanegas MA
AD  - Department of Neurology and Neurosurgery, Cone Laboratory and Center for Neuronal 
      Survival, Montreal Neurological Institute, McGill University, Montreal, Quebec 
      H3A 2B4, Canada.
FAU - Fawcett, J P
AU  - Fawcett JP
FAU - Causing, C G
AU  - Causing CG
FAU - Miller, F D
AU  - Miller FD
FAU - Sadikot, A F
AU  - Sadikot AF
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Comp Neurol
JT  - The Journal of comparative neurology
JID - 0406041
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - EC 1.14.17.1 (Dopamine beta-Hydroxylase)
RN  - VTD58H1Z2X (Dopamine)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/analysis/*genetics/physiology
MH  - Dopamine/*metabolism
MH  - Dopamine beta-Hydroxylase/analysis/*genetics/metabolism
MH  - Heterozygote
MH  - Humans
MH  - Immunohistochemistry
MH  - Mesencephalon/cytology/*physiology
MH  - Mice
MH  - Mice, Transgenic
MH  - Neurons/*cytology/*physiology
MH  - Promoter Regions, Genetic
EDAT- 1999/09/29 00:00
MHDA- 1999/09/29 00:01
CRDT- 1999/09/29 00:00
PHST- 1999/09/29 00:00 [pubmed]
PHST- 1999/09/29 00:01 [medline]
PHST- 1999/09/29 00:00 [entrez]
AID - 10.1002/(SICI)1096-9861(19991025)413:3<449::AID-CNE7>3.0.CO;2-2 [pii]
AID - 10.1002/(sici)1096-9861(19991025)413:3<449::aid-cne7>3.0.co;2-2 [doi]
PST - ppublish
SO  - J Comp Neurol. 1999 Oct 25;413(3):449-62. doi: 
      10.1002/(sici)1096-9861(19991025)413:3<449::aid-cne7>3.0.co;2-2.