PMID- 10502469
OWN - NLM
STAT- MEDLINE
DCOM- 19991019
LR  - 20061115
IS  - 0014-4894 (Print)
IS  - 0014-4894 (Linking)
VI  - 93
IP  - 2
DP  - 1999 Oct
TI  - Acanthocheilonema viteae: characterization of a molt-associated 
      excretory/secretory 18-kDa protein.
PG  - 73-81
AB  - Post-invasive third-stage larvae (pL3) of Acanthocheilonema viteae were labeled 
      with [(35)S]-methionine in vivo, and proteins released into the culture 
      supernatant before and during the third molt were analyzed. The molting 
      supernatant (MSN) contained abundant proteins of 14, 18, 29, and 36 kDa. The 14- 
      and 29-kDa proteins were exclusively found in the MSN, while the 18- and 36-kDa 
      proteins were also produced by nonmolting pL3, albeit in much lower quantities. 
      The cDNA for the most abundant protein in the MSN, an 18-kDa protein (Av18), was 
      isolated by polymerase chain reaction (PCR) with reverse transcribed (RT) RNA of 
      pL3, using information of the protein sequence. The Av18 full-length cDNA of 583 
      base pairs contained the 5' spliced leader sequence of nematodes, an open reading 
      frame of 427 base pairs, and a poly(A) tail in typical distance to a 
      polyadenylation signal. The deduced amino acid sequence encodes for a protein 
      with a calculated size of 15.8 kDa. The N-terminus starts with a hydrophobic 
      signal sequence and a predicted cleavage site after amino acid 20. The Av18 
      protein showed homologies to the deduced amino acid sequence of the larval 
      transcripts Bm-alt-1 and alt-2 of Brugia malayi and to the Dirofilaria immitis 
      proteins Di20/22 as well as to the Onchocerca volvulus proteins Ov-alt-1 and 
      Ov-alt-2. Av18 is present in all parasite stages within the mammalian host, as 
      determined by immunoblot with sera against the Escherichia coli-expressed protein 
      and RT-PCR experiments. However, it was released into culture medium only by L3 
      and adult female worms. In female worms Av18 was localized in the cuticular 
      region as demonstrated by immunofluorescent antibody tests using cryosections.
CI  - Copyright 1999 Academic Press.
FAU - Pogonka, T
AU  - Pogonka T
AD  - Department of Molecular Parasitology (Institute of Biology), Humboldt University 
      Berlin, Philippstrasse 13, Berlin, 10115, USA.
FAU - Oberlander, U
AU  - Oberlander U
FAU - Marti, T
AU  - Marti T
FAU - Lucius, R
AU  - Lucius R
LA  - eng
SI  - GENBANK/U27545
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Exp Parasitol
JT  - Experimental parasitology
JID - 0370713
RN  - 0 (Av18 protein, Acanthocheilonema viteae)
RN  - 0 (DNA, Complementary)
RN  - 0 (DNA, Helminth)
RN  - 0 (Helminth Proteins)
RN  - 0 (RNA, Helminth)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Blotting, Southern
MH  - Blotting, Western
MH  - DNA, Complementary/chemistry
MH  - DNA, Helminth/chemistry
MH  - Dipetalonema/*physiology
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Female
MH  - Fluorescent Antibody Technique, Indirect
MH  - Gerbillinae
MH  - Helminth Proteins/*chemistry/genetics
MH  - Male
MH  - Molecular Sequence Data
MH  - Molecular Weight
MH  - Molting/*physiology
MH  - Polymerase Chain Reaction
MH  - RNA, Helminth/genetics
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Sequence Alignment
MH  - Sequence Homology, Amino Acid
MH  - Species Specificity
MH  - Ticks
EDAT- 1999/09/30 00:00
MHDA- 1999/09/30 00:01
CRDT- 1999/09/30 00:00
PHST- 1999/09/30 00:00 [pubmed]
PHST- 1999/09/30 00:01 [medline]
PHST- 1999/09/30 00:00 [entrez]
AID - S0014-4894(99)94445-4 [pii]
AID - 10.1006/expr.1999.4445 [doi]
PST - ppublish
SO  - Exp Parasitol. 1999 Oct;93(2):73-81. doi: 10.1006/expr.1999.4445.