PMID- 10502729
OWN - NLM
STAT- MEDLINE
DCOM- 19991021
LR  - 20190708
IS  - 0020-7136 (Print)
IS  - 0020-7136 (Linking)
VI  - 84
IP  - 5
DP  - 1999 Oct 22
TI  - Genetic alterations in early-onset invasive breast carcinomas: correlation of 
      c-erbB-2 amplification detected by fluorescence in situ hybridization with p53 
      accumulation and tumor phenotype.
PG  - 511-5
AB  - p53 tumor-suppressor gene mutation and p53 protein over-expression have been 
      reported with higher frequency in early-onset breast carcinomas (EOBC). Given the 
      role attributed to normal p53 protein in DNA-repair mechanisms, other somatic 
      genomic alterations would be expected to be associated with this abnormality. 
      Amplification of the c-erbB-2 (HER-2/neu) oncogene and over-expression of the 
      corresponding p185erbB-2 protein have been linked to prognosis and response to 
      therapy in breast cancer. In a retrospective study of 62 formalin-fixed 
      paraffin-embedded invasive EOBC (diagnosed at 35 years or less), the 
      amplification status of the c-erbB-2 gene detected by fluorescence in situ 
      hybridization (FISH) using a unique sequence probe was compared with p53 protein 
      accumulation measured by immunohistochemistry (IHC) and phenotypic features. 
      p185erbB2-protein expression was also detected by immunohistochemistry, together 
      with estrogen-receptor (ER) and progesterone-receptor (PR) expression. The data 
      for a sub-set of 33 node-negative EOBC cases were compared with 70 node-negative 
      tumors diagnosed in women above 36 years of age. Compared with node-negative BC 
      in older women, node-negative EOBC was significantly more likely to feature high 
      grade, high proliferation rate, negative ER and/or PR and p53 over-expression (p 
      < 0.05). A trend toward a higher incidence of c-erbB-2 amplification in EOBC (21% 
      vs. 9%) reached near-significance (p = 0.07). In EOBC, c-erbB-2 amplification and 
      p53 over-expression were not associated with high tumor grade or high 
      cell-proliferation rate, in contrast to the significant associations of these 
      markers in tumors in older women. Abnormalities in tumor markers, including 
      c-erbB-2 gene amplification and p53-protein over-expression, occur at different 
      rates in women with EOBC as compared with BC developing in older women. This 
      finding may reflect a different pathogenesis for EOBC, and warrants further 
      investigation.
CI  - Copyright 1999 Wiley-Liss, Inc.
FAU - Fiche, M
AU  - Fiche M
AD  - University Hospital, Nantes, France.
FAU - Avet-Loiseau, H
AU  - Avet-Loiseau H
FAU - Heymann, M F
AU  - Heymann MF
FAU - Moussaly, F
AU  - Moussaly F
FAU - Digabel, C
AU  - Digabel C
FAU - Joubert, M
AU  - Joubert M
FAU - Classe, J M
AU  - Classe JM
FAU - Dravet, F
AU  - Dravet F
FAU - Fumoleau, P
AU  - Fumoleau P
FAU - Ross, J
AU  - Ross J
FAU - Maugard, C M
AU  - Maugard CM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Int J Cancer
JT  - International journal of cancer
JID - 0042124
RN  - 0 (Tumor Suppressor Protein p53)
RN  - EC 2.7.10.1 (Receptor, ErbB-2)
SB  - IM
MH  - Adult
MH  - Breast Neoplasms/*genetics
MH  - Female
MH  - *Gene Amplification
MH  - *Genes, erbB-2
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Middle Aged
MH  - Phenotype
MH  - Receptor, ErbB-2/analysis
MH  - Tumor Suppressor Protein p53/*analysis
EDAT- 1999/09/30 00:00
MHDA- 1999/09/30 00:01
CRDT- 1999/09/30 00:00
PHST- 1999/09/30 00:00 [pubmed]
PHST- 1999/09/30 00:01 [medline]
PHST- 1999/09/30 00:00 [entrez]
AID - 10.1002/(SICI)1097-0215(19991022)84:5<511::AID-IJC11>3.0.CO;2-5 [pii]
AID - 10.1002/(sici)1097-0215(19991022)84:5<511::aid-ijc11>3.0.co;2-5 [doi]
PST - ppublish
SO  - Int J Cancer. 1999 Oct 22;84(5):511-5. doi: 
      10.1002/(sici)1097-0215(19991022)84:5<511::aid-ijc11>3.0.co;2-5.