PMID- 10505605
OWN - NLM
STAT- MEDLINE
DCOM- 19991202
LR  - 20190701
IS  - 0304-3940 (Print)
IS  - 0304-3940 (Linking)
VI  - 272
IP  - 3
DP  - 1999 Sep 17
TI  - Intracerebroventricular administration of copper-zinc superoxide dismutase 
      inhibits pulsatile luteinizing hormone secretion in ovariectomized ewes.
PG  - 159-62
AB  - Intracerebroventricular (i.c.v.) injection of an inhibitor of nitric oxide 
      synthase (NOS) abolishes pulsatile luteinizing hormone (LH) secretion. It has 
      been demonstrated that structural and functional analogs of copper-zinc 
      superoxide dismutase (Cu,Zn-SOD) inhibit neuronal NOS. The present study examined 
      the ability of Cu,Zn-SOD to affect pulsatile LH release in the ewe. Bovine 
      Cu,Zn-SOD was administrated into the third cerebral ventricle of unanesthetized, 
      freely moving, ovariectomized (OVX) ewes. Jugular blood samples were taken every 
      15 min for 5 h before and 8 h after i.c.v. injections. In a pilot trial using 
      three OVX ewes, i.c.v. injection of Cu,Zn-SOD at a dose of 0.5, 1.0 or 2.0 microg 
      in 100 microl saline decreased plasma LH levels and abolished LH pulses, without 
      affecting FSH secretion. In the main experiment, i.c.v. injection of 100 microl 
      saline had no effect on mean LH levels and LH pulse frequency, whereas i.c.v. 
      injection of Cu,Zn-SOD at a dose of 1 microg/100 microl saline significantly (P < 
      0.01) decreased mean LH levels and LH pulse frequency. In conclusion, this study 
      provides the first evidence for the role of Cn,Zn-SOD in the control of LH 
      secretion at the level of the brain in female mammals.
FAU - Al-Gubory, K H
AU  - Al-Gubory KH
AD  - Institut National de la Recherche Agronomique, Unite de Recherches de Physiologie 
      Animale, Jouy-en-Josas, France. algubory@jouy.inra.fr
FAU - Locatelli, A
AU  - Locatelli A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Ireland
TA  - Neurosci Lett
JT  - Neuroscience letters
JID - 7600130
RN  - 9002-67-9 (Luteinizing Hormone)
RN  - 9002-68-0 (Follicle Stimulating Hormone)
RN  - EC 1.15.1.1 (Superoxide Dismutase)
SB  - IM
MH  - Animals
MH  - Depression, Chemical
MH  - Female
MH  - Follicle Stimulating Hormone/blood
MH  - Injections, Intraventricular
MH  - Luteinizing Hormone/*metabolism
MH  - Mice
MH  - *Ovariectomy
MH  - Sheep
MH  - Superoxide Dismutase/administration & dosage/*pharmacology
EDAT- 1999/10/03 00:00
MHDA- 1999/10/03 00:01
CRDT- 1999/10/03 00:00
PHST- 1999/10/03 00:00 [pubmed]
PHST- 1999/10/03 00:01 [medline]
PHST- 1999/10/03 00:00 [entrez]
AID - S0304394099006059 [pii]
AID - 10.1016/s0304-3940(99)00605-9 [doi]
PST - ppublish
SO  - Neurosci Lett. 1999 Sep 17;272(3):159-62. doi: 10.1016/s0304-3940(99)00605-9.