PMID- 10505762
OWN - NLM
STAT- MEDLINE
DCOM- 19991123
LR  - 20190905
IS  - 0885-3177 (Print)
IS  - 0885-3177 (Linking)
VI  - 19
IP  - 3
DP  - 1999 Oct
TI  - Pancreatic beta-cell replication in streptozotocin-diabetic rats: the effect of 
      liver compensatory growth on intraportally engrafted islets.
PG  - 304-9
AB  - Early studies showed that compensatory liver growth after anterior portal branch 
      ligation (aPBL) may restore normoglycemia in streptozotocin (STZ)-diabetic rats, 
      in which a subtherapeutic islet mass was previously transplanted into the liver. 
      We hypothesized that this effect could be related to islet regeneration at the 
      graft site. This study was designed to characterize the proliferative response of 
      the intraportally transplanted islets, shortly after aPBL. Male Wistar-Furth rats 
      were used as syngeneic islet donors and/or recipients. STZ-diabetic rats were 
      divided in four groups: groups 1 and 2 underwent selective 250-islet 
      transplantation (Tx) into the posterior liver lobes, followed by aPBL 10 days 
      later; rats were killed 24 h (n = 9) and 48 h (n = 10) after aPBL, respectively; 
      groups 3 and 4 underwent selective 250-islet Tx into the posterior liver lobes, 
      followed by sham aPBL 10 days later; rats were killed 24 h (n = 3) and 48 h (n = 
      3) after aPBL, respectively. Two hours before killing, all animals were injected 
      with 5'-bromo-2'-deoxyuridine (BrdU; 50 mg/kg, i.v.). Liver sections were 
      immunostained for insulin and BrdU, and both hepatocyte and islet cell labeling 
      index (LI) were calculated. Islet cell LI was 2.30+/-1.18% in group 1, 
      2.23+/-1.00% in group 2, 0.43+/-0.29% in group 3, and 0.39+/-0.21% in group 4 
      (group 1 vs. group 3: p<0.02; group 2 vs. group 4: p<0.01). Hepatocyte LI was 
      2.50+/-2.14% in group 1, 15.0+/-7.6% in group 2, 0.12 +/-0.04 in group 3, and 
      0.11+/-0.03% in group 4, respectively (group 1 vs. group 2: p<0.02; group 1 vs. 
      group 3: p<0.001; group 2 vs. group 4: p<0.001). Our study showed that 
      intraportally transplanted islets undergo a concurrent proliferative response 
      after aPBL, although with a lower extent and a different timing when compared 
      with the liver-cell response.
FAU - Fogli, L
AU  - Fogli L
AD  - Sant'Anna University Hospital, Ferrara, Italy. foglil@tin.it
FAU - Morsiani, E
AU  - Morsiani E
FAU - Bertanti, T
AU  - Bertanti T
FAU - Eguchi, S
AU  - Eguchi S
FAU - Azzena, G
AU  - Azzena G
FAU - Demetriou, A A
AU  - Demetriou AA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Pancreas
JT  - Pancreas
JID - 8608542
RN  - 0 (Blood Glucose)
RN  - G34N38R2N1 (Bromodeoxyuridine)
SB  - IM
MH  - Animals
MH  - Bile Ducts/cytology
MH  - Blood Glucose
MH  - Bromodeoxyuridine
MH  - Cell Division
MH  - Diabetes Mellitus, Experimental/blood/*pathology/therapy
MH  - Islets of Langerhans/*cytology
MH  - *Islets of Langerhans Transplantation
MH  - Ligation
MH  - Liver/*cytology
MH  - Liver Regeneration
MH  - Male
MH  - Pancreas/*cytology
MH  - Portal Vein
MH  - Rats
MH  - Rats, Inbred WF
EDAT- 1999/10/03 00:00
MHDA- 1999/10/03 00:01
CRDT- 1999/10/03 00:00
PHST- 1999/10/03 00:00 [pubmed]
PHST- 1999/10/03 00:01 [medline]
PHST- 1999/10/03 00:00 [entrez]
AID - 10.1097/00006676-199910000-00012 [doi]
PST - ppublish
SO  - Pancreas. 1999 Oct;19(3):304-9. doi: 10.1097/00006676-199910000-00012.