PMID- 10506104
OWN - NLM
STAT- MEDLINE
DCOM- 19991104
LR  - 20231213
IS  - 0143-3334 (Print)
IS  - 0143-3334 (Linking)
VI  - 20
IP  - 10
DP  - 1999 Oct
TI  - Gap junction proteins connexin32 and connexin43 partially acquire 
      growth-suppressive function in HeLa cells by deletion of their C-terminal tails.
PG  - 1913-8
AB  - Our laboratory has previously reported that transfection of a connexin26 (Cx26) 
      gene, but not connexin40 nor connexin43 (Cx43), into HeLa cells expressing no 
      detectable level of connexins suppressed the tumorigenic phenotype of the HeLa 
      cells both in vitro and in vivo, although all of these connexins induced gap 
      junctional intercellular communication in HeLa cells to a similar extent. The 
      most remarkable structural difference between connexin proteins is the length of 
      the C-terminal cytoplasmic tail, Cx26 having almost no tail, while Cx43 and 
      connexin32 (Cx32) have long and intermediate ones, respectively. When Cx32 and 
      Cx43 lose their C-terminal tails, they seem to resemble Cx26 in structure. To 
      examine whether such truncated connexins become tumor suppressive in HeLa cells, 
      we introduced a stop codon into each of the Cx32 and Cx43 cDNAs to remove their 
      C-terminal tails and transfected these constructs (DeltaCx) into HeLa cells. Both 
      DeltaCx cDNAs induced GJIC as efficiently as the wild-type counterparts. Although 
      none of the truncated connexins affected proliferation rate, the truncated Cx32 
      and Cx43 proteins suppressed anchorage-independent cell growth in soft agar. 
      Furthermore, when the transfectants were injected into the backs of nude mice, 
      tumor appearance was delayed by 7 days in animals given cells expressing 
      truncated connexins, i.e. tumors became detectable on days 11 and 18 after 
      injection of vector and DeltaCx transfectants, respectively. Although throughout 
      these experiments the truncated connexins did not completely eliminate the 
      tumorigenicity of HeLa cells, as Cx26 did, it was evident that deletion of the 
      C-terminal tails gave both Cx32 and Cx43 a capacity for negative growth control, 
      suggesting that the C-terminal tails of these two connexins function as a 
      regulatory region for connexin-mediated growth control in HeLa cells.
FAU - Omori, Y
AU  - Omori Y
AD  - Unit of Multistage Carcinogenesis, International Agency for Research on Cancer, 
      150 cours Albert-Thomas, 69372 Lyon Cedex 08, France.
FAU - Yamasaki, H
AU  - Yamasaki H
LA  - eng
GR  - R01-CA-40534/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Carcinogenesis
JT  - Carcinogenesis
JID - 8008055
RN  - 0 (Connexin 43)
RN  - 0 (Connexins)
RN  - 0 (GJB2 protein, human)
RN  - 127120-53-0 (Connexin 26)
SB  - IM
MH  - Animals
MH  - Cell Communication/genetics
MH  - Cell Division/*physiology
MH  - Connexin 26
MH  - Connexin 43/genetics/*physiology
MH  - Connexins/genetics/*physiology
MH  - HeLa Cells
MH  - Humans
MH  - Immunohistochemistry
MH  - Mice
MH  - Mice, Nude
MH  - Phenotype
MH  - Sequence Deletion
MH  - Transfection
MH  - Gap Junction beta-1 Protein
EDAT- 1999/10/03 00:00
MHDA- 1999/10/03 00:01
CRDT- 1999/10/03 00:00
PHST- 1999/10/03 00:00 [pubmed]
PHST- 1999/10/03 00:01 [medline]
PHST- 1999/10/03 00:00 [entrez]
AID - 10.1093/carcin/20.10.1913 [doi]
PST - ppublish
SO  - Carcinogenesis. 1999 Oct;20(10):1913-8. doi: 10.1093/carcin/20.10.1913.