PMID- 10507550
OWN - NLM
STAT- MEDLINE
DCOM- 19991124
LR  - 20190701
IS  - 0304-3940 (Print)
IS  - 0304-3940 (Linking)
VI  - 272
IP  - 2
DP  - 1999 Sep 10
TI  - In vivo activation and in situ BDNF-stimulated nuclear translocation of 
      mitogen-activated/extracellular signal-regulated protein kinase is inhibited by 
      ethanol in the developing rat hippocampus.
PG  - 95-8
AB  - In order to test the hypothesis that ethanol (EtOH)-induced changes in growth 
      factor signal transduction contribute to the teratogenic effects of EtOH in the 
      developing brain, neonatal rat pups were administered a single dose of EtOH 
      during the brain growth spurt (5 days of age, PN5). Hippocampal 
      mitogen-activated/extracellular signal-regulated protein kinase (MAPK/ERK) 
      activation was analyzed one to 6 h after exposure by electrophoretic-mobility 
      shift assay combined with western blot. Brain-Derived Neurotrophic Factor (BDNF) 
      was used to stimulate ERK in hippocampal slices prepared from PN5 pups and 
      activation and cellular localization was determined with immunofluorescence 
      combined with confocal microscopy. EtOH decreased ERK activation in vivo and 
      decreased nuclear translocation of BDNF-stimulated ERK in situ. These data 
      suggest EtOH-induced inhibition of growth factor signaling may contribute to the 
      development of fetal alcohol syndrome and alcohol-related birth defects.
FAU - Davis, M I
AU  - Davis MI
AD  - Department of Biomedical Sciences, School of Public Health at the University at 
      Albany, NY, USA. midavis@ufbi.ufl.edu
FAU - Szarowski, D
AU  - Szarowski D
FAU - Turner, J N
AU  - Turner JN
FAU - Morrisett, R A
AU  - Morrisett RA
FAU - Shain, W
AU  - Shain W
LA  - eng
GR  - AA05381/AA/NIAAA NIH HHS/United States
GR  - AA09230/AA/NIAAA NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Ireland
TA  - Neurosci Lett
JT  - Neuroscience letters
JID - 7600130
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 3K9958V90M (Ethanol)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinases)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/*metabolism
MH  - Cell Nucleus/drug effects
MH  - Ethanol/*pharmacology
MH  - Fluorescent Antibody Technique
MH  - Hippocampus/*drug effects/growth & development/*metabolism
MH  - Microscopy, Confocal
MH  - Mitogen-Activated Protein Kinases/*metabolism
MH  - Organ Culture Techniques
MH  - Phosphorylation/drug effects
MH  - Rats
EDAT- 1999/10/03 00:00
MHDA- 1999/10/03 00:01
CRDT- 1999/10/03 00:00
PHST- 1999/10/03 00:00 [pubmed]
PHST- 1999/10/03 00:01 [medline]
PHST- 1999/10/03 00:00 [entrez]
AID - S0304-3940(99)00572-8 [pii]
AID - 10.1016/s0304-3940(99)00572-8 [doi]
PST - ppublish
SO  - Neurosci Lett. 1999 Sep 10;272(2):95-8. doi: 10.1016/s0304-3940(99)00572-8.