PMID- 10510087
OWN - NLM
STAT- MEDLINE
DCOM- 19991104
LR  - 20190508
IS  - 0022-1007 (Print)
IS  - 1540-9538 (Electronic)
IS  - 0022-1007 (Linking)
VI  - 190
IP  - 7
DP  - 1999 Oct 4
TI  - Defective development of gamma/delta T cells in interleukin 7 receptor-deficient 
      mice is due to impaired expression of T cell receptor gamma genes.
PG  - 973-82
AB  - Mice lacking the interleukin 7 receptor (IL-7R) generate alpha/beta T cells at a 
      detectable but greatly reduced rate, but gamma/delta T cells are completely 
      absent. The special role of IL-7R signaling in gamma/delta T cell development has 
      remained unclear. IL-7Ralpha(-/-) mice exhibit a paucity of gamma gene 
      rearrangements. This striking observation can be explained by a defect in T cell 
      receptor (TCR)-gamma gene rearrangement, a defect in TCR-gamma gene transcription 
      leading to death of gamma/delta lineage cells, and/or a requirement for IL-7R in 
      commitment of cells to the gamma/delta lineage. To determine the role of IL-7R 
      signaling in gamma/delta T cell development, we examined transcription of a 
      prerearranged TCR-gamma transgene in IL-7Ralpha(-/-) mice, as well as the effects 
      of IL-7 on transcription of endogenous, rearranged TCR-gamma genes in alpha/beta 
      lineage cells. The results demonstrate that IL-7R-mediated signals are necessary 
      for the normal expression of rearranged TCR-gamma genes. Equally significant, the 
      results show that the poor expression of TCR-gamma genes in IL-7Ralpha(-/-) mice 
      is responsible for the selective deficit in gamma/delta cells in these mice, 
      since a high copy TCR-gamma transgene exhibited sufficient residual expression in 
      IL-7Ralpha(-/-) mice to drive gamma/delta cell development. The results indicate 
      that the absence of gamma/delta T cells in IL-7Ralpha(-/-) mice is due to 
      insufficient TCR-gamma gene expression.
FAU - Kang, J
AU  - Kang J
AD  - Department of Molecular and Cell Biology and the Cancer Research Laboratory, 
      Division of Immunology, University of California at Berkeley, Berkeley, 
      California 94720, USA.
FAU - Coles, M
AU  - Coles M
FAU - Raulet, D H
AU  - Raulet DH
LA  - eng
GR  - R01-AI30171/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Exp Med
JT  - The Journal of experimental medicine
JID - 2985109R
RN  - 0 (Receptors, Antigen, T-Cell, gamma-delta)
RN  - 0 (Receptors, Interleukin-7)
SB  - IM
MH  - Animals
MH  - Flow Cytometry
MH  - Gene Expression Regulation/immunology
MH  - *Gene Rearrangement, gamma-Chain T-Cell Antigen Receptor
MH  - *Genes, T-Cell Receptor gamma
MH  - Mice
MH  - Mice, Knockout
MH  - Mice, Transgenic
MH  - Receptors, Antigen, T-Cell, gamma-delta/*genetics/*immunology/physiology
MH  - Receptors, Interleukin-7/deficiency/genetics/*physiology
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Signal Transduction
MH  - Spleen/immunology
MH  - T-Lymphocytes/*immunology
MH  - Thymus Gland/immunology
MH  - Transcription, Genetic
PMC - PMC2195640
EDAT- 1999/10/06 00:00
MHDA- 1999/10/06 00:01
PMCR- 2000/04/04
CRDT- 1999/10/06 00:00
PHST- 1999/10/06 00:00 [pubmed]
PHST- 1999/10/06 00:01 [medline]
PHST- 1999/10/06 00:00 [entrez]
PHST- 2000/04/04 00:00 [pmc-release]
AID - 99-0576 [pii]
AID - 10.1084/jem.190.7.973 [doi]
PST - ppublish
SO  - J Exp Med. 1999 Oct 4;190(7):973-82. doi: 10.1084/jem.190.7.973.