PMID- 10510198
OWN - NLM
STAT- MEDLINE
DCOM- 19991124
LR  - 20231213
IS  - 0953-816X (Print)
IS  - 0953-816X (Linking)
VI  - 11
IP  - 9
DP  - 1999 Sep
TI  - Long-term changes in connexin32 gap junction protein and mRNA expression 
      following cocaine self-administration in rats.
PG  - 3329-38
AB  - Considerable evidence indicates a critical role for dopamine in the reinforcing 
      effects of cocaine. Because dopamine has been shown to be a critical modulator of 
      gap junction communication in both eye and brain, we sought to examine whether 
      extended intravenous cocaine self-administration would affect the expression of 
      gap junction channel-forming proteins (connexins). Using ELISA, Western analysis, 
      immunohistochemistry, semi-quantitative reverse transcriptase polymerase chain 
      reaction (RT-PCR), and non-radioactive in situ hybridization, we demonstrate that 
      withdrawal from chronic cocaine self-administration causes lasting changes in 
      connexin32 (Cx32) expression in the nucleus accumbens and hippocampus at 2, 7 and 
      21 days after the last cocaine injection. A sustained decrease in Cx32 protein 
      and mRNA levels is noted in areas that have been implicated in cocaine craving 
      (i.e. nucleus accumbens and subfields of the hippocampal formation). A 
      progressive increase in gap junction protein and mRNA expression is noted in 
      areas that become hyperexcitable after chronic cocaine exposure (i.e. CA1 
      hippocampal neurons). We speculate that gap junction communication may be 
      critically involved in reinforcement processes and neuroadaptive changes produced 
      by drugs of abuse.
FAU - Bennett, S A
AU  - Bennett SA
AD  - Institute of Biochemistry, Carleton University, Ottawa, ON, Canada.
FAU - Arnold, J M
AU  - Arnold JM
FAU - Chen, J
AU  - Chen J
FAU - Stenger, J
AU  - Stenger J
FAU - Paul, D L
AU  - Paul DL
FAU - Roberts, D C
AU  - Roberts DC
LA  - eng
GR  - GM37551/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - France
TA  - Eur J Neurosci
JT  - The European journal of neuroscience
JID - 8918110
RN  - 0 (Connexins)
RN  - 0 (Dopamine Uptake Inhibitors)
RN  - 0 (RNA, Messenger)
RN  - I5Y540LHVR (Cocaine)
SB  - IM
MH  - Animals
MH  - Antibody Specificity
MH  - Blotting, Western
MH  - Cocaine/adverse effects/*pharmacology
MH  - Connexins/*biosynthesis/immunology
MH  - Dopamine Uptake Inhibitors/adverse effects/*pharmacology
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Epilepsy/chemically induced
MH  - Gap Junctions/drug effects/*metabolism
MH  - Hippocampus/metabolism
MH  - Immunohistochemistry
MH  - In Situ Hybridization
MH  - Injections
MH  - Male
MH  - Neostriatum/metabolism
MH  - Nucleus Accumbens/metabolism
MH  - RNA, Messenger/*biosynthesis/genetics
MH  - Rats
MH  - Rats, Wistar
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Self Administration
MH  - Substance Withdrawal Syndrome/metabolism
MH  - Gap Junction beta-1 Protein
EDAT- 1999/10/06 00:00
MHDA- 1999/10/06 00:01
CRDT- 1999/10/06 00:00
PHST- 1999/10/06 00:00 [pubmed]
PHST- 1999/10/06 00:01 [medline]
PHST- 1999/10/06 00:00 [entrez]
AID - ejn752 [pii]
AID - 10.1046/j.1460-9568.1999.00752.x [doi]
PST - ppublish
SO  - Eur J Neurosci. 1999 Sep;11(9):3329-38. doi: 10.1046/j.1460-9568.1999.00752.x.