PMID- 10510330
OWN - NLM
STAT- MEDLINE
DCOM- 19991102
LR  - 20181113
IS  - 0021-9738 (Print)
IS  - 0021-9738 (Linking)
VI  - 104
IP  - 7
DP  - 1999 Oct
TI  - Increased cortical bone mineral content but unchanged trabecular bone mineral 
      density in female ERbeta(-/-) mice.
PG  - 895-901
AB  - Ovariectomy in young, growing rodents results in decreased trabecular bone 
      mineral density (BMD) and increased radial growth of the cortical bone. Both of 
      these effects are reversed by treatment with estrogen. The aim of the present 
      study was to determine the physiological role of estrogen receptor-beta (ERbeta) 
      on bone structure and bone mineral content (BMC). The BMC was increased in adult 
      (11 weeks old), but not prepubertal (4 weeks old), female ERbeta(-/-) mice 
      compared with wild-type (WT) mice. This increase in BMC in females was not due to 
      increased trabecular BMD, but to an increased cross-sectional cortical bone area 
      associated with a radial bone growth. Male ERbeta(-/-) mice displayed no bone 
      abnormalities compared with WT mice. Ovariectomy decreased the trabecular BMD to 
      the same extent in adult female ERbeta(-/-) mice as in WT mice. The expression 
      levels of osteoblast-associated genes - alpha1(I) collagen, alkaline phosphatase, 
      and osteocalcin mRNAs - were elevated in bone from adult ERbeta(-/-) females 
      compared with WT mice. These observations provide a possible explanation for the 
      increased radial bone growth seen in female mutants, suggesting a repressive 
      function for ERbeta in the regulation of bone growth during female adolescence. 
      In summary, ERbeta is essential for the pubertal feminization of the cortical 
      bone in female mice but is not required for the protective effect of estrogens on 
      trabecular BMD.
FAU - Windahl, S H
AU  - Windahl SH
AD  - Department of Biosciences, Karolinska Institute, Novum, S-14157 Huddinge, Sweden.
FAU - Vidal, O
AU  - Vidal O
FAU - Andersson, G
AU  - Andersson G
FAU - Gustafsson, J A
AU  - Gustafsson JA
FAU - Ohlsson, C
AU  - Ohlsson C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Clin Invest
JT  - The Journal of clinical investigation
JID - 7802877
RN  - 0 (Estrogen Receptor beta)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (Receptors, Estrogen)
RN  - 147205-72-9 (CD40 Ligand)
RN  - 9007-34-5 (Collagen)
RN  - EC 3.1.3.1 (Alkaline Phosphatase)
SB  - IM
MH  - Aging
MH  - Alkaline Phosphatase/genetics
MH  - Animals
MH  - Body Weight
MH  - Bone Density/*physiology
MH  - *Bone Development
MH  - CD40 Ligand
MH  - Collagen/genetics
MH  - Estrogen Receptor beta
MH  - Female
MH  - Femur/growth & development
MH  - Gene Expression Regulation, Developmental
MH  - Growth Plate/growth & development
MH  - Male
MH  - Membrane Glycoproteins/genetics
MH  - Mice
MH  - Mice, Knockout
MH  - Ovariectomy
MH  - Receptors, Estrogen/deficiency/genetics/*physiology
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Sex Characteristics
MH  - Sexual Maturation
MH  - Tibia/growth & development
PMC - PMC408552
EDAT- 1999/10/08 00:00
MHDA- 1999/10/08 00:01
PMCR- 1999/10/01
CRDT- 1999/10/08 00:00
PHST- 1999/10/08 00:00 [pubmed]
PHST- 1999/10/08 00:01 [medline]
PHST- 1999/10/08 00:00 [entrez]
PHST- 1999/10/01 00:00 [pmc-release]
AID - 06730 [pii]
AID - 10.1172/JCI6730 [doi]
PST - ppublish
SO  - J Clin Invest. 1999 Oct;104(7):895-901. doi: 10.1172/JCI6730.