PMID- 10510791
OWN - NLM
STAT- MEDLINE
DCOM- 19991025
LR  - 20190831
IS  - 1387-2273 (Print)
IS  - 1387-2273 (Linking)
VI  - 731
IP  - 2
DP  - 1999 Aug 20
TI  - Simultaneous determination of catecholamines in rat brain tissue by 
      high-performance liquid chromatography.
PG  - 361-7
AB  - A novel and highly sensitive method has been developed for the determination of 
      catecholamines [noradrenaline (NA), dopamine (DA), serotonin (5-HT) and their 
      metabolites 5-hydroxyindoleacetic acid (5-HIAA) and homovanillic acid (HVA)] in 
      brain tissue. The method uses isocratic reversed-phase HPLC with amperometric 
      end-point detection. The calibration curve was linear over the range 10-150 pg 
      on-column. The assay limits of detection for NA, DA, 5-HT, 5-HIAA and HVA were 
      3.8, 3.8, 6.8, 5 and 7.5 pg on-column, respectively. The mean inter- and 
      intra-assay relative standard deviations (RSDs) over the range of the standard 
      curve were less than 5%. The absolute recoveries averaged 99.1%, 99.5%, 97.7%, 
      99.5% and 98.8% for NA, DA, 5-HT, 5-HIAA and HVA, respectively.
FAU - Chi, J D
AU  - Chi JD
AD  - University of Oxford, Department of Psychiatry, Warneford Hospital, Headington, 
      UK.
FAU - Odontiadis, J
AU  - Odontiadis J
FAU - Franklin, M
AU  - Franklin M
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - J Chromatogr B Biomed Sci Appl
JT  - Journal of chromatography. B, Biomedical sciences and applications
JID - 9714109
RN  - 0 (Catecholamines)
SB  - IM
MH  - Animals
MH  - *Brain Chemistry
MH  - Catecholamines/*analysis
MH  - Chromatography, High Pressure Liquid/*methods
MH  - Electrochemistry
MH  - Rats
MH  - Reproducibility of Results
MH  - Sensitivity and Specificity
EDAT- 1999/10/08 00:00
MHDA- 1999/10/08 00:01
CRDT- 1999/10/08 00:00
PHST- 1999/10/08 00:00 [pubmed]
PHST- 1999/10/08 00:01 [medline]
PHST- 1999/10/08 00:00 [entrez]
AID - 10.1016/s0378-4347(99)00255-8 [doi]
PST - ppublish
SO  - J Chromatogr B Biomed Sci Appl. 1999 Aug 20;731(2):361-7. doi: 
      10.1016/s0378-4347(99)00255-8.