PMID- 10512985
OWN - NLM
STAT- MEDLINE
DCOM- 19991126
LR  - 20220310
IS  - 0022-3034 (Print)
IS  - 0022-3034 (Linking)
VI  - 41
IP  - 2
DP  - 1999 Nov 5
TI  - Neurotrophic factor regulation of developing avian oculomotor neurons: 
      differential effects of BDNF and GDNF.
PG  - 295-315
AB  - Neurotrophic factors support the development of motoneurons by several possible 
      mechanisms. Neurotrophins may act as target-derived factors or as afferent 
      factors derived from the central nervous system (CNS) or sensory ganglia. We 
      tested whether brain-derived neurotrophic factor (BDNF), neurotrophin 3 (NT-3), 
      neurotrophin 4 (NT-4), and glial cell line-derived neurotrophic factor (GDNF) may 
      be target-derived factors for neurons in the oculomotor (MIII) or trochlear (MIV) 
      nucleus in chick embryos. Radio-iodinated BDNF, NT-3, NT-4, and GDNF accumulated 
      in oculomotor neurons via retrograde axonal transport when the trophic factors 
      were applied to the target. Systemic GDNF rescued oculomotor neurons from 
      developmental cell death, while BDNF and NT-3 had no effect. BDNF enhanced 
      neurite outgrowth from explants of MIII and MIV nuclei (identified by retrograde 
      labeling in ovo with the fluorescent tracer DiI), while GDNF, NT-3, and NT-4 had 
      no effect. The oculomotor neurons were immunoreactive for BDNF and the BDNF 
      receptors p75(NTR) and trkB. To determine whether BDNF may be derived from its 
      target or may act as an autocrine or paracrine factor, in situ hybridization and 
      deprivation studies were performed. BDNF mRNA expression was detected in eye 
      muscles, but not in CNS sources of afferent innervation to MIII, or the 
      oculomotor complex itself. Injection of trkB fusion proteins in the eye muscle 
      reduced BDNF immunoreactivity in the innervating motoneurons. These data indicate 
      that BDNF trophic support for the oculomotor neurons was derived from their 
      target.
CI  - Copyright 1999 John Wiley & Sons, Inc.
FAU - Steljes, T P
AU  - Steljes TP
AD  - Department of Physiology and Cell Biology, Mailstop 352, University of Nevada 
      School of Medicine, Reno, Nevada 89557, USA.
FAU - Kinoshita, Y
AU  - Kinoshita Y
FAU - Wheeler, E F
AU  - Wheeler EF
FAU - Oppenheim, R W
AU  - Oppenheim RW
FAU - von Bartheld, C S
AU  - von Bartheld CS
LA  - eng
GR  - GM 8194-16/GM/NIGMS NIH HHS/United States
GR  - NS 20402/NS/NINDS NIH HHS/United States
GR  - NS 31380/NS/NINDS NIH HHS/United States
GR  - etc.
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Neurobiol
JT  - Journal of neurobiology
JID - 0213640
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Glial Cell Line-Derived Neurotrophic Factor)
RN  - 0 (Iodine Radioisotopes)
RN  - 0 (Nerve Growth Factors)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Neurotrophin 3)
RN  - 0 (RNA, Messenger)
RN  - 0 (Recombinant Fusion Proteins)
RN  - EC 2.7.10.1 (Receptor, trkB)
RN  - P658DCA9XD (neurotrophin 4)
SB  - IM
MH  - Animals
MH  - Axonal Transport/physiology
MH  - Brain-Derived Neurotrophic Factor/analysis/*pharmacokinetics
MH  - Cell Death/drug effects
MH  - Cells, Cultured
MH  - Chick Embryo
MH  - Glial Cell Line-Derived Neurotrophic Factor
MH  - In Situ Hybridization
MH  - Iodine Radioisotopes/pharmacokinetics
MH  - Motor Neurons/cytology/*physiology/ultrastructure
MH  - Nerve Growth Factors/pharmacokinetics
MH  - Nerve Tissue Proteins/*pharmacokinetics
MH  - Neurites/chemistry/physiology
MH  - Neurotrophin 3/pharmacokinetics
MH  - Oculomotor Nerve/*cytology/embryology
MH  - RNA, Messenger/analysis
MH  - Receptor, trkB/analysis/genetics
MH  - Recombinant Fusion Proteins/analysis/pharmacokinetics
MH  - Trochlear Nerve/*cytology/embryology
EDAT- 1999/10/08 00:00
MHDA- 1999/10/08 00:01
CRDT- 1999/10/08 00:00
PHST- 1999/10/08 00:00 [pubmed]
PHST- 1999/10/08 00:01 [medline]
PHST- 1999/10/08 00:00 [entrez]
AID - 10.1002/(SICI)1097-4695(19991105)41:2<295::AID-NEU11>3.0.CO;2-W [pii]
PST - ppublish
SO  - J Neurobiol. 1999 Nov 5;41(2):295-315.