PMID- 10513004
OWN - NLM
STAT- MEDLINE
DCOM- 19991126
LR  - 20190729
IS  - 1060-3271 (Print)
IS  - 1060-3271 (Linking)
VI  - 82
IP  - 5
DP  - 1999 Sep-Oct
TI  - Liquid chromatographic determination of fenbendazole residues in pig tissues 
      after treatment with medicated feed.
PG  - 1007-16
AB  - Fenbendazole (FBZ) is an anthelmintic widely used in farm animals to treat 
      parasitic infestations. In pigs, it is administered in the food. The aim of this 
      study was to validate an analytical method for the determination of FBZ and its 
      metabolites in pig tissues. This method is based on oxidation of FBZ and its 
      sulfoxide metabolite to the sulfone metabolite (FBZSO2). The limit of 
      quantitation for this method is 20 ng FBZSO2/g for all tissues. The maximum 
      residue limits (MRLs) established for FBZ and its metabolites in pig tissues are 
      50 ng/g for muscle, fat, and kidney and 500 ng/g for liver. This method is based 
      on a liquid-liquid extraction followed by an oxidation with peracetic acid and a 
      cleanup procedure based on 2 liquid-liquid extractions. Determination is achieved 
      by high-performance liquid chromatography with ultraviolet detection. The present 
      method is adjusted to the MRL established for FBZ and its metabolite residues. 
      The analysis of the residues shows that after 72 h posttreatment, no FBZSO2 was 
      detected in muscle, fat, and kidney and that liver levels were below the MRL.
FAU - Capece, B P
AU  - Capece BP
AD  - Unitat de Farmacologia i de Terapeutica, Facultat de Veterinaria, UAB, 
      Bellaterra, Spain.
FAU - Perez, B
AU  - Perez B
FAU - Castells, E
AU  - Castells E
FAU - Arboix, M
AU  - Arboix M
FAU - Cristofol, C
AU  - Cristofol C
LA  - eng
PT  - Journal Article
PL  - England
TA  - J AOAC Int
JT  - Journal of AOAC International
JID - 9215446
RN  - 0 (Antinematodal Agents)
RN  - 621BVT9M36 (Fenbendazole)
SB  - IM
MH  - Adipose Tissue/chemistry
MH  - Animal Feed
MH  - Animals
MH  - Antinematodal Agents/*analysis
MH  - Calibration
MH  - Chromatography, Liquid
MH  - Drug Residues/*analysis
MH  - Fenbendazole/*analysis
MH  - Kidney/chemistry
MH  - Linear Models
MH  - Liver/chemistry
MH  - Male
MH  - Muscles/chemistry
MH  - Reproducibility of Results
MH  - Sensitivity and Specificity
MH  - Swine
EDAT- 1999/10/08 00:00
MHDA- 1999/10/08 00:01
CRDT- 1999/10/08 00:00
PHST- 1999/10/08 00:00 [pubmed]
PHST- 1999/10/08 00:01 [medline]
PHST- 1999/10/08 00:00 [entrez]
PST - ppublish
SO  - J AOAC Int. 1999 Sep-Oct;82(5):1007-16.