PMID- 10513576
OWN - NLM
STAT- MEDLINE
DCOM- 19991101
LR  - 20190624
IS  - 0014-2999 (Print)
IS  - 0014-2999 (Linking)
VI  - 380
IP  - 2-3
DP  - 1999 Sep 10
TI  - 5-hydroxytryptamine induces transient Ca2+ influx through Ni2+-insensitive Ca2+ 
      channels in rat vascular smooth muscle cells.
PG  - 163-70
AB  - The effects of Ni2+, a non-selective cation channel inhibitor, on 
      5-hydroxytryptamine (5-HT)- and angiotensin II (Ang II)-induced intracellular 
      Ca2+ dynamics in rat aortic smooth muscle cells were investigated. Ni2+ (1 mM) 
      significantly inhibited the transient increase in intracellular Ca2+ 
      concentration ([Ca2+]i) induced by Ang II (100 nM) in aortic smooth muscle cells, 
      as measured using fura-2. However, Ni2+ did not suppress the transient increase 
      in Ca2+ influx induced by 5-HT (10 microM), while significantly suppressed the 
      sustained increase. Ca2+ influx evoked by high KCl (80 mM), thapsigargin (TG) (1 
      microM) or depletion of intracellular Ca2+ store was almost completely suppressed 
      by Ni2+. Ni2+ had no effect on 5-HT-induced inositol triphosphate production and 
      Ca2+ release from the intracellular store(s). These results suggest that 5-HT, 
      but not Ang II, induces transient Ca2+ influx through Ni2+-insensitive Ca2+ 
      channels, which are distinguishable from the voltage-dependent or store-operated 
      Ca2+ channels.
FAU - Hirafuji, M
AU  - Hirafuji M
AD  - Department of Pharmacology, Faculty of Pharmaceutical Sciences, Health Sciences 
      University of Hokkaido, Ishikari-Tobetsu, Japan. hirafuji@hoku-iryo-u.ac.jp
FAU - Kawahara, F
AU  - Kawahara F
FAU - Ebihara, T
AU  - Ebihara T
FAU - Nezu, A
AU  - Nezu A
FAU - Tanimura, A
AU  - Tanimura A
FAU - Minami, M
AU  - Minami M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Eur J Pharmacol
JT  - European journal of pharmacology
JID - 1254354
RN  - 0 (Calcium Channels)
RN  - 11128-99-7 (Angiotensin II)
RN  - 333DO1RDJY (Serotonin)
RN  - 660YQ98I10 (Potassium Chloride)
RN  - 67526-95-8 (Thapsigargin)
RN  - 7OV03QG267 (Nickel)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Angiotensin II/pharmacology
MH  - Animals
MH  - Calcium/*metabolism/pharmacology
MH  - Calcium Channels/*drug effects
MH  - Dose-Response Relationship, Drug
MH  - Male
MH  - Muscle, Smooth, Vascular/cytology/*drug effects/metabolism
MH  - Nickel/*pharmacology
MH  - Potassium Chloride/pharmacology
MH  - Rats
MH  - Rats, Wistar
MH  - Serotonin/*pharmacology
MH  - Thapsigargin/pharmacology
EDAT- 1999/10/08 00:00
MHDA- 1999/10/08 00:01
CRDT- 1999/10/08 00:00
PHST- 1999/10/08 00:00 [pubmed]
PHST- 1999/10/08 00:01 [medline]
PHST- 1999/10/08 00:00 [entrez]
AID - S0014-2999(99)00532-4 [pii]
AID - 10.1016/s0014-2999(99)00532-4 [doi]
PST - ppublish
SO  - Eur J Pharmacol. 1999 Sep 10;380(2-3):163-70. doi: 10.1016/s0014-2999(99)00532-4.