PMID- 10513602
OWN - NLM
STAT- MEDLINE
DCOM- 19991102
LR  - 20191210
IS  - 0304-4920 (Print)
IS  - 0304-4920 (Linking)
VI  - 42
IP  - 2
DP  - 1999 Jun 30
TI  - Corticotropin-releasing factor enhances brain-derived neurotrophic factor gene 
      expression to facilitate memory retention in rats.
PG  - 73-81
AB  - In the present study, we investigated the effects of corticotropin-releasing 
      factor (CRF) injected into the dentate gyrus (DG) of the hippocampus on 
      brain-derived neurotrophic factor (BDNF) mRNA expression and studied whether 
      N-methyl-D-aspartate (NMDA) receptor mediates the effects of CRF on BDNF mRNA 
      expression in the DG. Since both CRF and BDNF gene expressions are involved in 
      memory processing in rats, we further investigated whether CRF facilitates memory 
      retention through enhanced BDNF mRNA expression in the hippocampus. Effect of 
      direct BDNF injection to the DG on retention performance in rats was also 
      assessed. Results indicated that intra-DG CRF injection produced a dose-dependent 
      (0.1 microg, 1.0 microg and 10 microg) increase in BDNF mRNA level, while 
      intra-DG MK801 injection produced a dose-dependent (0.08 microg, 0.2 microg and 
      2.0 microg) decrease in BDNF mRNA expression in the DG. MK801, at a dose having 
      no significant effect alone (0.08 microg), significantly antagonized the effect 
      of CRF on BDNF mRNA expression. On the other hand, CRF (1.0 microg) consistently 
      and markedly improved retention performance in rats in an inhibitory avoidance 
      learning task. BDNF antisense oligonucleotide treatment, at a concentration which 
      did not affect retention performance alone (0.5 mM), blocked the memory-enhancing 
      effect of CRF. However, direct and chronic BDNF injection to the DG did not 
      improve memory performance in rats. These results together suggest that at least 
      one of the mechanisms responsible for the memory-facilitating effect of CRF is 
      mediated through enhanced BDNF mRNA expression in the hippocampus. The lack of an 
      effect of intra-DG BDNF injection on memory retention is also discussed.
FAU - Ma, Y L
AU  - Ma YL
AD  - Institute of Biomedical Sciences, Academia Sinica, Taipei, Taiwan, ROC.
FAU - Chen, K Y
AU  - Chen KY
FAU - Wei, C L
AU  - Wei CL
FAU - Lee, E H
AU  - Lee EH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - India
TA  - Chin J Physiol
JT  - The Chinese journal of physiology
JID - 7804502
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (RNA, Messenger)
RN  - 6LR8C1B66Q (Dizocilpine Maleate)
RN  - 9015-71-8 (Corticotropin-Releasing Hormone)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/*genetics/pharmacology
MH  - Corticotropin-Releasing Hormone/antagonists & inhibitors/*pharmacology
MH  - Dentate Gyrus/metabolism/physiology
MH  - Dizocilpine Maleate/pharmacology
MH  - Dose-Response Relationship, Drug
MH  - Gene Expression/*drug effects
MH  - Injections
MH  - Male
MH  - RNA, Messenger/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Retention, Psychology/*drug effects/*physiology
EDAT- 1999/10/08 00:00
MHDA- 1999/10/08 00:01
CRDT- 1999/10/08 00:00
PHST- 1999/10/08 00:00 [pubmed]
PHST- 1999/10/08 00:01 [medline]
PHST- 1999/10/08 00:00 [entrez]
PST - ppublish
SO  - Chin J Physiol. 1999 Jun 30;42(2):73-81.