PMID- 10514401
OWN - NLM
STAT- MEDLINE
DCOM- 19991104
LR  - 20181113
IS  - 0002-9440 (Print)
IS  - 1525-2191 (Electronic)
IS  - 0002-9440 (Linking)
VI  - 155
IP  - 4
DP  - 1999 Oct
TI  - Abundant production of brain-derived neurotrophic factor by adult visceral 
      epithelia. Implications for paracrine and target-derived Neurotrophic functions.
PG  - 1183-93
AB  - Brain-derived neurotrophic factor (BDNF) plays a crucial role for the survival of 
      visceral sensory neurons during development. However, the physiological sources 
      and the function of BDNF in the adult viscera are poorly described. We have 
      investigated the cellular sources and the potential role of BDNF in adult murine 
      viscera. We found markedly different amounts of BDNF protein in different organs. 
      Surprisingly, BDNF levels in the urinary bladder, lung, and colon were higher 
      than those found in the brain or skin. In situ hybridization experiments revealed 
      that BDNF mRNA was made by visceral epithelial cells, several types of smooth 
      muscle, and neurons of the myenteric plexus. Epithelia that expressed BDNF lacked 
      both the high- and low-affinity receptors for BDNF, trkB and p75(NTR). In 
      contrast, both receptors were present on neurons of the peripheral nervous 
      system. Studies with BDNF-/-mice demonstrated that epithelial and smooth muscle 
      cells developed normally in the absence of BDNF. These data provide evidence that 
      visceral epithelia are a major source, but not a target, of BDNF in the adult 
      viscera. The abundance of BDNF protein in certain internal organs suggests that 
      this neurotrophin may regulate the function of adult visceral sensory and motor 
      neurons.
FAU - Lommatzsch, M
AU  - Lommatzsch M
AD  - Department of Laboratory Medicine, Charite, Humboldt University, Berlin, Germany.
FAU - Braun, A
AU  - Braun A
FAU - Mannsfeldt, A
AU  - Mannsfeldt A
FAU - Botchkarev, V A
AU  - Botchkarev VA
FAU - Botchkareva, N V
AU  - Botchkareva NV
FAU - Paus, R
AU  - Paus R
FAU - Fischer, A
AU  - Fischer A
FAU - Lewin, G R
AU  - Lewin GR
FAU - Renz, H
AU  - Renz H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Am J Pathol
JT  - The American journal of pathology
JID - 0370502
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptor, Nerve Growth Factor)
RN  - EC 2.7.10.1 (Receptor, trkB)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/*biosynthesis/blood/physiology/urine
MH  - Cardiovascular System/metabolism
MH  - Digestive System/metabolism
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Epithelial Cells/metabolism
MH  - Female
MH  - In Situ Hybridization
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Knockout
MH  - Paracrine Communication/physiology
MH  - RNA, Messenger/biosynthesis
MH  - Receptor, Nerve Growth Factor/metabolism
MH  - Receptor, trkB/metabolism
MH  - Respiratory System/metabolism
MH  - Urogenital System/metabolism
MH  - Viscera/*metabolism
PMC - PMC1867012
EDAT- 1999/10/09 00:00
MHDA- 1999/10/09 00:01
PMCR- 2000/04/01
CRDT- 1999/10/09 00:00
PHST- 1999/10/09 00:00 [pubmed]
PHST- 1999/10/09 00:01 [medline]
PHST- 1999/10/09 00:00 [entrez]
PHST- 2000/04/01 00:00 [pmc-release]
AID - S0002-9440(10)65221-2 [pii]
AID - 1902 [pii]
AID - 10.1016/S0002-9440(10)65221-2 [doi]
PST - ppublish
SO  - Am J Pathol. 1999 Oct;155(4):1183-93. doi: 10.1016/S0002-9440(10)65221-2.