PMID- 10515254
OWN - NLM
STAT- MEDLINE
DCOM- 19991110
LR  - 20190915
IS  - 0168-0102 (Print)
IS  - 0168-0102 (Linking)
VI  - 34
IP  - 3
DP  - 1999 Aug
TI  - Deprived of habitual running, rats downregulate BDNF and TrkB messages in the 
      brain.
PG  - 125-32
AB  - To study possible effects of physical training on the expression of neurotrophic 
      factors and their receptors in the brain, we used a rat strain (spontaneously 
      hypertensive rat, SHR), known to spontaneously run up to 20 km/night. We show 
      that such long-distance running affects the brain-derived neurotrophic factor 
      (BDNF) and TrkB system in hippocampus, and in particular that abrupt deprivation 
      of habitual running leads to long-lasting decreases of BDNF/TrkB expression in 
      hippocampus. Quantitative in situ hybridization demonstrates that running 
      increases the expression of mRNA coding for BDNF and its high affinity receptor 
      TrkB in hippocampus in a running length dependent manner. In addition, we show 
      that an abrupt interruption of prolonged spontaneous exercise decrease expression 
      of mRNA encoding BDNF and TrkB in certain hippocampal areas and that this 
      decrease lasts at least 10 days. This down-regulation was most prominent in 
      medial cornu ammonis 3 (CA3M). Several other trophic factors and receptors were 
      investigated, including NGF, NT3, GDNF, trkC and p75. For these other probes 
      investigated, no robust changes in mRNA expression were noted. Areas examined 
      included sensorimotor cortex and hippocampus. For RET, p75, NT3, TrkB and BDNF we 
      also examined the spinal cord without detecting any robust changes. We conclude 
      that spontaneous running as well as its abrupt termination, leads to 
      area-specific and trophic factor-specific changes in hippocampus.
FAU - Widenfalk, J
AU  - Widenfalk J
AD  - Department of Neuroscience, Karolinska Institute, Stockholm, Sweden. 
      johan.widenfalk@neuro.ki.se
FAU - Olson, L
AU  - Olson L
FAU - Thoren, P
AU  - Thoren P
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Ireland
TA  - Neurosci Res
JT  - Neuroscience research
JID - 8500749
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (RNA, Messenger)
RN  - EC 2.7.10.1 (Receptor, trkB)
SB  - IM
MH  - Animals
MH  - Brain/*metabolism
MH  - Brain-Derived Neurotrophic Factor/*genetics
MH  - Cerebral Cortex/metabolism
MH  - Down-Regulation
MH  - Gene Expression Regulation
MH  - Hippocampus/metabolism
MH  - In Situ Hybridization
MH  - Physical Exertion/*physiology
MH  - RNA, Messenger/*genetics/metabolism
MH  - Rats
MH  - Rats, Inbred SHR
MH  - Receptor, trkB/*genetics
MH  - Running/physiology
MH  - Spinal Cord/metabolism
EDAT- 1999/10/09 00:00
MHDA- 1999/10/09 00:01
CRDT- 1999/10/09 00:00
PHST- 1999/10/09 00:00 [pubmed]
PHST- 1999/10/09 00:01 [medline]
PHST- 1999/10/09 00:00 [entrez]
AID - S0168-0102(99)00051-6 [pii]
AID - 10.1016/s0168-0102(99)00051-6 [doi]
PST - ppublish
SO  - Neurosci Res. 1999 Aug;34(3):125-32. doi: 10.1016/s0168-0102(99)00051-6.