PMID- 10515604
OWN - NLM
STAT- MEDLINE
DCOM- 20000113
LR  - 20240315
IS  - 1016-8478 (Print)
IS  - 1016-8478 (Linking)
VI  - 9
IP  - 4
DP  - 1999 Aug 31
TI  - Chromosomal localization of ESTs obtained from human fetal liver via BAC-mediated 
      FISH mapping.
PG  - 403-9
AB  - A total of 55 expressed sequence tags (ESTs) randomly chosen from our collection 
      of fetal liver ESTs were mapped to chromosomes by fluorescence in situ 
      hybridization (FISH) mapping techniques. To generate FISH mapping probes, the 
      genomic DNAs for each EST were selected by screening an arrayed human bacterial 
      artificial chromosome (BAC) library. In total, 73 BACs were used for mapping of 
      the 55 ESTs. Among them, 70 BACs representing 52 ESTs unequivocally mapped to 
      single chromosomal regions. The remaining 3 BACs representing 3 ESTs were 
      localized to multiple regions, suggesting that BACs may have very low chimerism. 
      Our mapping results were compared with EST mapping databases deposited in NCBI. 
      Thirty-six of 55 ESTs corresponded to previously mapped positions of ESTs, 2 ESTs 
      mapped to different positions from previously determined ones, and it was found 
      that 17 ESTs have been mapped on new locations from this study. These mapping 
      data may be used for completing the framework of the human physical map, and also 
      for providing a good starting point for searching disease-related genes.
FAU - Choi, S S
AU  - Choi SS
AD  - Department of Life Science, Pohang University of Science and Technology, Korea.
FAU - Kang, Y S
AU  - Kang YS
FAU - Kim, U J
AU  - Kim UJ
FAU - Lee, K H
AU  - Lee KH
FAU - Shin, H S
AU  - Shin HS
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Mol Cells
JT  - Molecules and cells
JID - 9610936
RN  - 0 (DNA, Complementary)
SB  - IM
MH  - Blotting, Southern
MH  - *Chromosome Mapping
MH  - Chromosomes, Bacterial/*genetics
MH  - DNA, Complementary/analysis
MH  - Databases, Factual
MH  - *Expressed Sequence Tags
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Liver/*embryology/*metabolism
MH  - Nucleic Acid Hybridization
MH  - Physical Chromosome Mapping
MH  - Reproducibility of Results
MH  - Sequence Tagged Sites
EDAT- 1999/10/09 00:00
MHDA- 1999/10/09 00:01
CRDT- 1999/10/09 00:00
PHST- 1999/10/09 00:00 [pubmed]
PHST- 1999/10/09 00:01 [medline]
PHST- 1999/10/09 00:00 [entrez]
AID - S1016-8478(23)13560-6 [pii]
PST - ppublish
SO  - Mol Cells. 1999 Aug 31;9(4):403-9.