PMID- 10515807
OWN - NLM
STAT- MEDLINE
DCOM- 19991209
LR  - 20071115
IS  - 0022-1899 (Print)
IS  - 0022-1899 (Linking)
VI  - 180
IP  - 5
DP  - 1999 Nov
TI  - Quantitative proviral DNA and antibody levels in the natural history of HTLV-I 
      infection.
PG  - 1487-93
AB  - The pathogenesis of human T-cell lymphotropic virus type I (HTLV-I) in adult 
      T-cell leukemia/lymphoma (ATL) and HTLV-I associated myelopathy/tropical spastic 
      paraparesis (HAM/TSP) is poorly understood. We prospectively followed up and 
      evaluated the virologic correlates of infection in transfusion recipients after 
      seroconversion, in asymptomatic carriers, and in ATL and HAM/TSP patients. 
      Proviral DNA levels (copies/105 lymphocytes) were determined by real-time 
      automated polymerase chain reaction and antibody titers by end-point dilution by 
      use of an HTLV-I enzyme-linked immunoassay. In early infection, proviral load was 
      initially elevated (median, 212 copies/105 lymphocytes at time 1) and later 
      decreased (median, 99 copies at time 2, and 27 copies at time 3). Corresponding 
      antibody titers were low at time 1 (1:2154), had significantly increased by time 
      2 (1:12312), and were stable by time 3 (1:4694). These viral markers were 
      significantly lower in asymptomatic carriers than in HAM/TSP or ATL patients. 
      Therefore, proviral load and antibody titers may be useful as predictive markers 
      of disease among carriers.
FAU - Manns, A
AU  - Manns A
AD  - Viral Epidemiology Branch, Division of Cancer Epidemiology and Genetics, National 
      Cancer Institute, Bethesda, MD 20852, USA. mannsa@epndce.nci.nih.gov
FAU - Miley, W J
AU  - Miley WJ
FAU - Wilks, R J
AU  - Wilks RJ
FAU - Morgan, O S
AU  - Morgan OS
FAU - Hanchard, B
AU  - Hanchard B
FAU - Wharfe, G
AU  - Wharfe G
FAU - Cranston, B
AU  - Cranston B
FAU - Maloney, E
AU  - Maloney E
FAU - Welles, S L
AU  - Welles SL
FAU - Blattner, W A
AU  - Blattner WA
FAU - Waters, D
AU  - Waters D
LA  - eng
GR  - N01-CP-40548/CP/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Infect Dis
JT  - The Journal of infectious diseases
JID - 0413675
RN  - 0 (DNA, Viral)
RN  - 0 (HTLV-I Antibodies)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Blood Transfusion
MH  - Carrier State/immunology/virology
MH  - DNA, Viral/*blood
MH  - Disease Progression
MH  - Female
MH  - HTLV-I Antibodies/*blood
MH  - HTLV-I Infections/*immunology/*virology
MH  - Human T-lymphotropic virus 1/immunology/*physiology
MH  - Humans
MH  - Leukemia-Lymphoma, Adult T-Cell/virology
MH  - Male
MH  - Middle Aged
MH  - Paraparesis, Tropical Spastic/virology
MH  - Pregnancy
MH  - Pregnancy Complications, Infectious/virology
MH  - Prospective Studies
MH  - *Proviruses
MH  - Viral Load
EDAT- 1999/10/09 09:00
MHDA- 2000/07/06 11:00
CRDT- 1999/10/09 09:00
PHST- 1999/10/09 09:00 [pubmed]
PHST- 2000/07/06 11:00 [medline]
PHST- 1999/10/09 09:00 [entrez]
AID - JID990358 [pii]
AID - 10.1086/315088 [doi]
PST - ppublish
SO  - J Infect Dis. 1999 Nov;180(5):1487-93. doi: 10.1086/315088.