PMID- 10516280
OWN - NLM
STAT- MEDLINE
DCOM- 19991117
LR  - 20180925
IS  - 0002-9513 (Print)
IS  - 0002-9513 (Linking)
VI  - 277
IP  - 4
DP  - 1999 Oct
TI  - Nitric oxide and renal nerve-mediated proximal tubular reabsorption in 
      normotensive and hypertensive rats.
PG  - F560-6
LID - 10.1152/ajprenal.1999.277.4.F560 [doi]
AB  - In Inactin-anesthetized Wistar rats with an intact renal innervation, 
      intratubular nitro-L-arginine methyl ester (L-NAME, 10(-4) M) increased proximal 
      fluid uptake (J(va), at 2.47 +/- 0.61 x 10(-4) mm(3). mm(-2). s(-1)) by 17% (P < 
      0.05), whereas coadministration with sodium nitroprusside (SNP, 10(-4) M) 
      decreased J(va) by 18% (P < 0.01). Similar manipulation of NO generation was 
      without effect in groups of Wistar rats subjected to acute renal denervation. 
      Intratubular aminoguanidine (10(-4) M), a selective inducible nitric oxide 
      synthase (NOS) blocker, had no effect on J(va) in intact kidneys of Wistar rats, 
      but the neuronal NOS (nNOS) blocker, 7-nitroindazole (10(-4) M and 10(-6) M) 
      increased J(va) by 19-23% (both P < 0.001). In stroke-prone spontaneously 
      hypertensive rats (SHRSP), J(va) values in the innervated kidneys were lower (P < 
      0.05) than in the corresponding Wistar groups and were unchanged by intratubular 
      L-NAME or L-NAME plus SNP. The tonic attenuation of proximal epithelial transport 
      by NO was dependent on the renal sympathetic nerves and appeared to be generated 
      by the nNOS isoform of the enzyme. This role of NO was not evident in the SHRSP.
FAU - Wu, X C
AU  - Wu XC
AD  - Department of Physiology, University of Birmingham, Birmingham B15 2TT, United 
      Kingdom.
FAU - Harris, P J
AU  - Harris PJ
FAU - Johns, E J
AU  - Johns EJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Am J Physiol
JT  - The American journal of physiology
JID - 0370511
RN  - 31C4KY9ESH (Nitric Oxide)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase Type I)
RN  - EC 1.14.13.39 (Nos1 protein, rat)
SB  - IM
MH  - Absorption/physiology
MH  - Animals
MH  - Genetic Predisposition to Disease
MH  - Hypertension/genetics/*metabolism
MH  - Kidney/*innervation
MH  - Kidney Tubules, Proximal/*metabolism
MH  - Male
MH  - Nervous System/physiopathology
MH  - Nitric Oxide/*physiology
MH  - Nitric Oxide Synthase/physiology
MH  - Nitric Oxide Synthase Type I
MH  - Rats
MH  - Rats, Inbred SHR/genetics
MH  - Rats, Wistar
MH  - Reference Values
MH  - Stroke/genetics
EDAT- 1999/10/12 00:00
MHDA- 1999/10/12 00:01
CRDT- 1999/10/12 00:00
PHST- 1999/10/12 00:00 [pubmed]
PHST- 1999/10/12 00:01 [medline]
PHST- 1999/10/12 00:00 [entrez]
AID - 10.1152/ajprenal.1999.277.4.F560 [doi]
PST - ppublish
SO  - Am J Physiol. 1999 Oct;277(4):F560-6. doi: 10.1152/ajprenal.1999.277.4.F560.