PMID- 10516433
OWN - NLM
STAT- MEDLINE
DCOM- 19991027
LR  - 20171101
IS  - 0301-0171 (Print)
IS  - 0301-0171 (Linking)
VI  - 86
IP  - 1
DP  - 1999
TI  - Structural unbalanced chromosome rearrangements resolved by comparative genomic 
      hybridization.
PG  - 51-5
AB  - The identification of unbalanced structural chromosome rearrangements using 
      conventional cytogenetic techniques depends on recognition of the unknown 
      material from its banding pattern. Even with optimally banded chromosomes, when 
      large chromosome segments are involved, cytogeneticists may not always be able to 
      determine the origin of extrachromosomal material and supernumerary chromosomes. 
      We report here on the application of comparative genomic hybridization (CGH), a 
      new molecular-cytogenetic assay capable of detecting chromosomal gains and 
      losses, to six clinical samples suspected of harboring unbalanced structural 
      chromosome abnormalities. CGH provided essential information on the nature of the 
      unbalanced aberration investigated in five of the six samples. This approach has 
      proved its ability to resolve complex karyotypes and to provide information when 
      metaphase chromosomes are not available. In cases where metaphase chromosome 
      spreads were available, confirmation of CGH results was easily obtained by 
      fluorescence in situ hybridization (FISH) using specific probes. Thus the 
      combined use of CGH and FISH provided an efficient method for resolving the 
      origin of aberrant chromosomal material unidentified by conventional cytogenetic 
      analysis.
FAU - Daniely, M
AU  - Daniely M
AD  - Institute of Human Genetics, Sheba Medical Center, Ramat-Gan, Israel.
FAU - Barkai, G
AU  - Barkai G
FAU - Goldman, B
AU  - Goldman B
FAU - Aviram-Goldring, A
AU  - Aviram-Goldring A
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - Switzerland
TA  - Cytogenet Cell Genet
JT  - Cytogenetics and cell genetics
JID - 0367735
SB  - IM
MH  - Abortion, Spontaneous
MH  - Chromosome Aberrations/*genetics
MH  - Chromosome Banding
MH  - Chromosome Deletion
MH  - Chromosome Inversion
MH  - Chromosome Painting
MH  - Chromosomes, Human/*genetics
MH  - *Cytogenetic Analysis
MH  - Female
MH  - Fetus/cytology/metabolism
MH  - Humans
MH  - In Situ Hybridization/*methods
MH  - Karyotyping
MH  - Male
MH  - Metaphase
MH  - Mutagenesis, Insertional/genetics
MH  - Pregnancy
MH  - Sensitivity and Specificity
MH  - Translocation, Genetic/genetics
EDAT- 1999/10/12 00:00
MHDA- 1999/10/12 00:01
CRDT- 1999/10/12 00:00
PHST- 1999/10/12 00:00 [pubmed]
PHST- 1999/10/12 00:01 [medline]
PHST- 1999/10/12 00:00 [entrez]
AID - 15409 [pii]
AID - 10.1159/000015409 [doi]
PST - ppublish
SO  - Cytogenet Cell Genet. 1999;86(1):51-5. doi: 10.1159/000015409.