PMID- 10517317
OWN - NLM
STAT- MEDLINE
DCOM- 19991101
LR  - 20211203
IS  - 0026-8925 (Print)
IS  - 0026-8925 (Linking)
VI  - 262
IP  - 2
DP  - 1999 Sep
TI  - Identification, characterization and chromosomal localization of the cognate 
      human and murine DBF4 genes.
PG  - 220-9
AB  - The kinase Dbf4p/Cdc7p is required for the G1/S phase transition during the cell 
      cycle and plays a direct role in the activation of individual origins of 
      replication in Saccharomyces cerevisiae. Here, we report the identification and 
      characterization of mouse and human cDNAs whose products are related in sequence 
      to Saccharomyces cerevisiae DBF4 cDNA. Both mammalian Dbf4 proteins contain a 
      putative site for phosphorylation by CDK, PEST protease cleavage sites, nuclear 
      localization signals and a short-looped zinc finger-like domain. Transcription of 
      MmDBF4 is suppressed in mouse NIH3T3 fibroblasts made quiescent by serum 
      starvation. Upon replenishment of the medium, transcript levels increase during 
      progression through G1, peaking as cells enter S phase. MmDbf4p interacts 
      physically with Cdc7p and Mcm2p in vivo. Using fluorescence in situ hybridization 
      (FISH), the human DBF4 gene was localized to chromosome 7 (q21.3), whereas FISH 
      mapped the murine counterpart to band A2 on chromosome 5. The results of 
      chromosome mapping indicate that in both mouse and human the gene is present as a 
      single copy. The structural conservation between Dbf4-related proteins suggests 
      that these proteins play a key role in the regulation of DNA replication during 
      the cell cycle in all eukaryotes.
FAU - Lepke, M
AU  - Lepke M
AD  - Institute of Biochemistry, Biozentrum, University of Wurzburg, Germany.
FAU - Putter, V
AU  - Putter V
FAU - Staib, C
AU  - Staib C
FAU - Kneissl, M
AU  - Kneissl M
FAU - Berger, C
AU  - Berger C
FAU - Hoehn, K
AU  - Hoehn K
FAU - Nanda, I
AU  - Nanda I
FAU - Schmid, M
AU  - Schmid M
FAU - Grummt, F
AU  - Grummt F
LA  - eng
SI  - GENBANK/AJ003132
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Mol Gen Genet
JT  - Molecular & general genetics : MGG
JID - 0125036
RN  - 0 (Cell Cycle Proteins)
RN  - 0 (DBF4 protein, human)
RN  - 0 (DBF4 protein, mouse)
RN  - 0 (DNA, Complementary)
RN  - 0 (Dbf4 protein, S cerevisiae)
RN  - 0 (Fungal Proteins)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (Saccharomyces cerevisiae Proteins)
RN  - EC 2.7.1.- (CDC7 protein, S cerevisiae)
RN  - EC 2.7.1.- (CDC7 protein, human)
RN  - EC 2.7.1.- (Cdc7 protein, mouse)
RN  - EC 2.7.11.1 (Protein Serine-Threonine Kinases)
RN  - EC 3.6.4.12 (Minichromosome Maintenance Complex Component 2)
SB  - IM
MH  - 3T3 Cells
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Cell Cycle Proteins/*genetics/metabolism
MH  - Chromosome Mapping
MH  - *Chromosomes, Human, Pair 7
MH  - DNA Replication
MH  - DNA, Complementary
MH  - Fungal Proteins
MH  - Humans
MH  - Mice
MH  - Minichromosome Maintenance Complex Component 2
MH  - Molecular Sequence Data
MH  - Nuclear Proteins/genetics/metabolism
MH  - Protein Serine-Threonine Kinases/genetics/metabolism
MH  - Recombinant Fusion Proteins/genetics/metabolism
MH  - *Saccharomyces cerevisiae Proteins
MH  - Sequence Homology, Amino Acid
EDAT- 1999/10/12 00:00
MHDA- 1999/10/12 00:01
CRDT- 1999/10/12 00:00
PHST- 1999/10/12 00:00 [pubmed]
PHST- 1999/10/12 00:01 [medline]
PHST- 1999/10/12 00:00 [entrez]
AID - 10.1007/s004380051078 [doi]
PST - ppublish
SO  - Mol Gen Genet. 1999 Sep;262(2):220-9. doi: 10.1007/s004380051078.