PMID- 10517515
OWN - NLM
STAT- MEDLINE
DCOM- 19991206
LR  - 20191024
IS  - 0141-8130 (Print)
IS  - 0141-8130 (Linking)
VI  - 26
IP  - 2-3
DP  - 1999 Nov
TI  - Mechanism of salmon sperm decondensation by nucleoplasmin.
PG  - 95-101
AB  - Removal of protamine from DNA-protamine (salmine, protamine from salmon sperm) 
      complexes by nucleoplasmin was examined and compared with that of poly-L-glutamic 
      acid (PLGA) using turbidity and ethidium bromide (EB) treatment methods. When 
      nucleoplasmin or PLGA was added to a DNA-protamine complex solution, turbidity 
      was decreased and the amount of EB intercalated into DNA was increased. These 
      results suggest that nucleoplasmin and PLGA can remove protamine from 
      DNA-protamine complexes. The effect of nucleoplasmin was more potent than that of 
      PLGA. Direct interaction of nucleoplasmin with protamine was confirmed by mixing 
      experiments using circular dichroism (CD) and fluorescence spectroscopies. 
      Results suggest that nucleoplasmin is bound to protamine in a 1:1 ratio and that 
      Trp126 is located near a hydrophilic region containing a polyglutamic acid tract 
      of nucleoplasmin which was obviously influenced by its binding with protamine. It 
      would appear that the polyglutamic acid tract in nucleoplasmin plays a critical 
      role for binding with protamine.
FAU - Iwata, K
AU  - Iwata K
AD  - Division of Bioscience, Graduate School of Environmental Earth Science, Hokkaido 
      University, Sapporo, Japan.
FAU - Hozumi, K
AU  - Hozumi K
FAU - Iihara, A
AU  - Iihara A
FAU - Nomizu, M
AU  - Nomizu M
FAU - Sakairi, N
AU  - Sakairi N
FAU - Nishi, N
AU  - Nishi N
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - Netherlands
TA  - Int J Biol Macromol
JT  - International journal of biological macromolecules
JID - 7909578
RN  - 0 (Nuclear Proteins)
RN  - 0 (Nucleoplasmins)
RN  - 0 (Phosphoproteins)
RN  - 0 (Protamines)
RN  - 0 (Solutions)
RN  - 25513-46-6 (Polyglutamic Acid)
RN  - 9007-49-2 (DNA)
RN  - EN464416SI (Ethidium)
SB  - IM
MH  - Animals
MH  - Circular Dichroism
MH  - DNA/drug effects/*metabolism
MH  - Ethidium/chemistry
MH  - Male
MH  - Nuclear Proteins/*metabolism/pharmacology
MH  - Nucleoplasmins
MH  - Phosphoproteins/*metabolism/pharmacology
MH  - Polyglutamic Acid/pharmacology
MH  - Protamines/drug effects/isolation & purification/*metabolism
MH  - Salmon
MH  - Solutions
MH  - Spectrometry, Fluorescence
MH  - Spermatozoa/drug effects/*physiology
EDAT- 1999/10/12 00:00
MHDA- 1999/10/12 00:01
CRDT- 1999/10/12 00:00
PHST- 1999/10/12 00:00 [pubmed]
PHST- 1999/10/12 00:01 [medline]
PHST- 1999/10/12 00:00 [entrez]
AID - S0141-8130(99)00071-9 [pii]
AID - 10.1016/s0141-8130(99)00071-9 [doi]
PST - ppublish
SO  - Int J Biol Macromol. 1999 Nov;26(2-3):95-101. doi: 10.1016/s0141-8130(99)00071-9.